Recombinant Mouse IL-7 Protein Summary
| Description |
Recombinant bioactive protein containing 130 amino acids for Mouse IL-7 Source:E. coli Amino Acid Sequence:(P10168) |
Preparation Method |
Determined to be >98% pure by SDS-PAGE |
| Details of Functionality |
The ED50 as determined by the dose-dependent stimulation of the proliferation of murine 2E8 cells is <0.2 ng/ml, corresponding to a specific activity of >5 x 106 units/mg. |
| Source |
E. coli |
| Protein/Peptide Type |
Recombinant Protein |
| Gene |
IL7 |
| Purity |
>98%, by SDS-PAGE |
| Endotoxin Note |
<0.1 ng/ug |
Applications/Dilutions
| Dilutions |
|
| Theoretical MW |
15 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer |
Sterile filtered and lyophilized with no additives |
| Concentration |
Lyoph |
| Purity |
>98%, by SDS-PAGE |
| Reconstitution Instructions |
Reconstitute in H2O to a concentration of 0.1 - 1.0 ug/ul. The solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for long-term storage. |
Alternate Names for Recombinant Mouse IL-7 Protein
Background
Interleukin-7 (IL-7) is a potent lymphoid cell growth factor that affects Pre-B, Pro-B and early T cells. It will also affect mature T cells in combination with other factors such as ConA and IL-2. Human and murine IL-7 is cross-species reactive. The recombinant Murine IL-7 is a 15.0 kDa protein containing 130 amino acid residues.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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