Human, Mouse IL-7 ELISA Kit (Colorimetric)

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ELISA: IL-7 ELISA Kit [Biotin] [KA1067] - Standard curve

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      KA1067
    • Availability
      Product Discontinued

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Human, Mouse IL-7 ELISA Kit (Colorimetric) Summary

Description
Quality control test: Standard curve. Mouse Il7 ELISA kit is a sandwich enzyme immunoassay for the quantitative measurement of mouse Il7.
Standard Curve Range
15.6 to 1000 pg/mL
Sensitivity
<1 pg/mL
Assay Type
Sandwich ELISA
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IL7

Applications/Dilutions

Dilutions
  • ELISA
  • Quantification
Application Notes
Useful for quantification.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Kit Components

Components
  1. ABC diluent buffer
  2. Antibody diluent buffer
  3. Avidin-Biotin-Peroxidase Complex (ABC), dilution 1:100
  4. Biotinylated anti-mouse IL-7 antibody, dilution 1:100
  5. Lyophilized recombinant mouse IL-7 standard
  6. Plate precoated with anti-mouse IL-7 antibody
  7. Sample diluent buffer
  8. TMB color developing agent
  9. TMB stop solution

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human, Mouse IL-7 ELISA Kit (Colorimetric)

  • IL7
  • IL-7
  • IL-7interleukin-7
  • interleukin 7
  • Lymphopoietin-1
  • PBGF

Background

Interleukin-7 (IL-7, previously known as lymphopoietin-1 and pre-B-cell growth factor) is a hematopoietic growth factor that stimulates early B and T cells. It can also co-stimulate the proliferation of mature T cells with other factors such as ConA (concanavalin A) and IL-2. IL-7 can induce the formation of lymphokine-activated killer (LAK) cells, it also plays a role in the development of cytotoxic T lymphocytes (CTL). IL-7 can stimulate the production of pro-inflammatory cytokines and enhance the tumoricidal activity of monocytes/macrophages. It is produced by thymic stromal cells, spleen cells and keratinocytes. Human and murine IL-7 show cross-species reactivity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for IL-7 ELISA Kit (KA1067). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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Bioinformatics

Gene Symbol IL7