IL-7 Antibody [Unconjugated]

Western blot shows 25 ng of Recombinant Human IL-7 (207-IL), Recombinant Mouse IL-7 (407-ML) and Recombinant Rat IL-7 (7857-RL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human IL-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-207-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody HAF109). A specific band was detected for IL-7 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Recombinant Human IL‑7 (Catalog # 207-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑7 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Human IL‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-207-NA). The ND50 is typically 0.075-0.5 µg/mL.

• Reactivity: Hu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-7 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human IL-7
  Asp26-His177
  Accession # P13232
• Specificity: Detects human IL-7 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL7
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.02-0.04 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-7 Antibody [Unconjugated]

• IL7
• IL-7
• IL-7interleukin-7
• interleukin 7
• Lymphopoietin-1
• PBGF

Background

IL-7, previously known as pre-B cell growth factor and lymphopoietin-1, was originally purified on the basis of its ability to promote the proliferation of precursor B cells. It has now been shown that IL-7 can also stimulate the proliferation of thymocytes, T cell progenitors and mature CD4⁺ and CD8⁺ T cells. IL-7 can induce the formation of lymphokine-activated killer (LAK) cells as well as the development of cytotoxic T lymphocytes (CTL). IL-7 was also shown to induce the V(D)J rearrangement of the T cell receptor beta gene in mouse fetal thymocytes. Among myeloid lineage cells, IL-7 can up-regulate the production of pro-inflammatory cytokines and stimulate the tumoricidal activity of monocytes/macrophages. ­IL-7 is expressed by adherent stromal cells from various tissues.

Human IL-7 cDNA encodes a precursor protein of 177 amino residues containing a 25 amino acid residue signal peptide. Mouse IL-7 has approximately 65% amino acid sequence identity with human IL-7 and both proteins exhibit cross-species activity.

IL-7 bioactivites are mediated by the binding of IL-7 to functional high-affinity receptor complexes. The ligand binding subunit (IL-7 R) of the IL-7 receptor complex has been cloned from human and mouse sources. In addition to the membrane-anchored form of the IL-7 receptor, a human cDNA clone that encodes a soluble form of the IL-7 R has also been isolated. The gamma chain of the IL-2 receptor complex has been shown to be an essential component for IL-7 signal transduction. Both IL-7 R and IL-2 R gamma  are members of the hematopoietin receptor superfamily. Cells known to express IL-7 receptors include pre-B cells, T cells and bone marrow cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-7 Antibody (AF-207-NA)(4)

Publications using AF-207-NA

• Normanton M, Alvarenga H, Hamerschlak N, Ribeiro A, Kondo A, Rizzo L, Marti L Interleukin 7 plays a role in T lymphocyte apoptosis inhibition driven by mesenchymal stem cell without favoring proliferation and cytokines secretion. PLoS ONE, 2014-09-03;9(9):e106673. 2014-09-03 [PMID: 25184791] (Neutralization, Human)
• Johnson SE, Shah N, Panoskaltsis-Mortari A, Lebien TW Murine and human IL-7 activate STAT5 and induce proliferation of normal human pro-B cells. J. Immunol., 2005-12-01;175(11):7325-31. 2005-12-01 [PMID: 16301638] (Neutralization, Human)
• Vasir B, Avigan D, Wu Z, Crawford K, Turnquist S, Ren J, Kufe D Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism. J. Immunol., 2005-02-15;174(4):2376-86. 2005-02-15 [PMID: 15699174] (Neutralization, Human)
• vanderSpek JC, Sutherland JA, Gill BM, Gorgun G, Foss FM, Murphy JR Structure function analysis of interleukin 7: requirement for an aromatic ring at position 143 of helix D. Cytokine, 2002-03-07;17(5):227-33. 2002-03-07 [PMID: 12027403] (Western Blot, Human)

Bioinformatics

• Gene Symbol: IL7
• Uniprot:
  • P13232()