IL-7 Antibody (AH34-12)

Western Blot: IL-7 Antibody (AH34-12) [NBP2-67277] - Analysis of IL7 on human thymus cell lysates using anti- IL7 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: IL-7 Antibody (AH34-12) [NBP2-67277] - Staining IL7 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: IL-7 Antibody (AH34-12) [NBP2-67277] - Analysis of paraffin-embedded rat spleen tissue using anti-IL7 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: IL-7 Antibody (AH34-12) [NBP2-67277] - Staining IL7 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: IL-7 Antibody (AH34-12) [NBP2-67277] - Staining IL7 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: IL-7 Antibody (AH34-12) [NBP2-67277] - Staining IL7 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: IL-7 Antibody (AH34-12) [NBP2-67277] - Analysis of paraffin-embedded human colon cancer tissue using anti-IL7 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: IL-7 Antibody (AH34-12) [NBP2-67277] - Analysis of paraffin-embedded human spleen tissue using anti-IL7 antibody. Counter stained with hematoxylin.

Product Discontinued

IL-7 Antibody (AH34-12) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein
• Localization: Secreted.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL7
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 1% BSA, 40% Glycerol
• Preservative: 0.2% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein A purified

Alternate Names for IL-7 Antibody (AH34-12)

• IL7
• IL-7
• IL-7interleukin-7
• interleukin 7
• Lymphopoietin-1
• PBGF

Background

Interleukin-7 (IL-7, previously known as lymphopoietin-1 and pre-B-cell growth factor) is a hematopoietic growth factor that stimulates early B and T cells. It can also co-stimulate the proliferation of mature T cells with other factors such as ConA (concanavalin A) and IL-2. IL-7 can induce the formation of lymphokine-activated killer (LAK) cells, it also plays a role in the development of cytotoxic T lymphocytes (CTL). IL-7 can stimulate the production of pro-inflammatory cytokines and enhance the tumoricidal activity of monocytes/macrophages. It is produced by thymic stromal cells, spleen cells and keratinocytes. Human and murine IL-7 show cross-species reactivity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL7