IL-7 Antibody (7417) [Unconjugated]

Recombinant Human IL‑7 (Catalog # 207-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑7 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑7 Monoclonal Antibody (Catalog # MAB207). The ND50 is typically 0.4-0.8 µg/mL.

• Reactivity: Hu
• Applications: WB, B/N, ELISA(Cap)
• Clone: 7417
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-7 Antibody (7417) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human IL-7
  Accession # P13232
• Specificity: Detects human IL-7 in Western blots. In ELISAs, does not cross-react with bovine FGF acidic, recombinant human (rh) G-CSF, rhGM-CSF, rhIL-1 alpha , rhIL-1 beta , rhIL-2, rhIL-3, rhIL-4, rhIL-6, recombinant mouse IL-7, rhIL-8, porcine PDGF, rhTNF-alpha , rhTNF-beta , human TGF-beta 1, or porcine TGF-beta 1.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL7
• Purity Statement: Protein A or G purified from ascites
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.4-0.8 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: ELISA Capture: Human IL-7 Antibody (Catalog # MAB207)
  ELISA Detection: Human IL-7 Biotinylated Antibody (Catalog # BAF207)
  Standard: Recombinant Human IL-7 (Catalog # 207-IL)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-7 Antibody (7417) [Unconjugated]

• IL7
• IL-7
• IL-7interleukin-7
• interleukin 7
• Lymphopoietin-1
• PBGF

Background

IL-7, previously known as pre-B cell growth factor and lymphopoietin-1, was originally purified on the basis of its ability to promote the proliferation of precursor B cells. It has now been shown that IL-7 can also stimulate the proliferation of thymocytes, T cell progenitors and mature CD4⁺ and CD8⁺ T cells. IL-7 can induce the formation of lymphokine-activated killer (LAK) cells as well as the development of cytotoxic T lymphocytes (CTL). IL-7 was also shown to induce the V(D)J rearrangement of the T cell receptor beta gene in mouse fetal thymocytes. Among myeloid lineage cells, IL-7 can up-regulate the production of pro-inflammatory cytokines and stimulate the tumoricidal activity of monocytes/macrophages. ­IL-7 is expressed by adherent stromal cells from various tissues.

Human IL-7 cDNA encodes a precursor protein of 177 amino residues containing a 25 amino acid residue signal peptide. Mouse IL-7 has approximately 65% amino acid sequence identity with human IL-7 and both proteins exhibit cross-species activity.

IL-7 bioactivites are mediated by the binding of IL-7 to functional high-affinity receptor complexes. The ligand binding subunit (IL-7 R) of the IL-7 receptor complex has been cloned from human and mouse sources. In addition to the membrane-anchored form of the IL-7 receptor, a human cDNA clone that encodes a soluble form of the IL-7 R has also been isolated. The gamma chain of the IL-2 receptor complex has been shown to be an essential component for IL-7 signal transduction. Both IL-7 R and IL-2 R gamma  are members of the hematopoietin receptor superfamily. Cells known to express IL-7 receptors include pre-B cells, T cells, and bone marrow cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-7 Antibody (MAB207)(18)

Publications using MAB207

• Sabrina Rueschenbaum, Chengcong Cai, Matthias Schmidt, Katharina Schwarzkopf, Ulf Dittmer, Stefan Zeuzem et al. Translation of IRF-1 Restricts Hepatic Interleukin-7 Production to Types I and II Interferons: Implications for Hepatic Immunity Frontiers in Immunology 1/14/2021 [PMID: 33584648]
• SJ Kim, HJ Chang, MV Volin, S Umar, K Van Raemdo, A Chevalier, K Palasiewic, JW Christman, S Volkov, S Arami, M Maz, A Mehta, RK Zomorrodi, DA Fox, N Sweiss, S Shahrara Macrophages are the primary effector cells in IL-7-induced arthritis Cell. Mol. Immunol., 2019-06-13;0(0):. 2019-06-13 [PMID: 31197255] (Flow Cytometry, Neutralization, Human)
• T Murakami, J Kim, Y Li, GE Green, A Shikanov, A Ono Secondary lymphoid organ fibroblastic reticular cells mediate trans-infection of HIV-1 via CD44-hyaluronan interactions Nat Commun, 2018-06-22;9(1):2436. 2018-06-22 [PMID: 29934525] (Flow Cytometry, Human)
• Ming Zeng, Ashley T. Haase Ex vivo Co-culture of Lymphoid Tissue Stromal Cells and T Cells Bio Protoc 9/5/2012 [PMID: 27709118]
• Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling Sci Rep, 2016-09-02;6(0):32320. 2016-09-02 [PMID: 27586092] (Flow Cytometry, Neutralization, Human)
• ANNIE BARTLETT, ANDREW J. SANDERS, FIONA RUGE, KEITH G. HARDING, WEN G. JIANG Potential implications of interleukin-7 in chronic wound healing Experimental and Therapeutic Medicine 2016-07-01 [PMID: 27347014] (Immunohistochemistry, Human)
• ANNIE BARTLETT, ANDREW J. SANDERS, FIONA RUGE, KEITH G. HARDING, WEN G. JIANG Potential implications of interleukin-7 in chronic wound healing Experimental and Therapeutic Medicine 7/1/2016 [PMID: 27347014]
• Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum. J Biol Chem, 2015-07-16;290(37):22338-51. 2015-07-16 [PMID: 26183780] (Neutralization, Human)
• Austin V. Stone, Kadie S. Vanderman, Jeffrey S. Willey, David L. Long, Thomas C. Register, Carol A. Shively et al. Osteoarthritic changes in vervet monkey knees correlate with meniscus degradation and increased matrix metalloproteinase and cytokine secretion Osteoarthritis and Cartilage 10/1/2015 [PMID: 26033163]
• Sarah M Churchman, Jehan J El-Jawhari, Agata N Burska, Rekha Parmar, Vincent Goëb, Philip G Conaghan et al. Modulation of peripheral T-cell function by interleukin-7 in rheumatoid arthritis Arthritis Research & Therapy 12/23/2014 [PMID: 25533722]
• Chen Z, Kim S, Chamberlain N, Pickens S, Volin M, Volkov S, Arami S, Christman J, Prabhakar B, Swedler W, Mehta A, Sweiss N, Shahrara S The novel role of IL-7 ligation to IL-7 receptor in myeloid cells of rheumatoid arthritis and collagen-induced arthritis. J Immunol, 2013-04-19;190(10):5256-66. 2013-04-19 [PMID: 23606539] (Neutralization, Human)
• Ming Zeng, Peter J. Southern, Cavan S. Reilly, Greg J. Beilman, Jeffrey G. Chipman, Timothy W. Schacker et al. Lymphoid Tissue Damage in HIV-1 Infection Depletes Naïve T Cells and Limits T Cell Reconstitution after Antiretroviral Therapy PLoS Pathogens 1/5/2012 [PMID: 22241988]
• Ming Zeng, Peter J. Southern, Cavan S. Reilly, Greg J. Beilman, Jeffrey G. Chipman, Timothy W. Schacker et al. Lymphoid Tissue Damage in HIV-1 Infection Depletes Naïve T Cells and Limits T Cell Reconstitution after Antiretroviral Therapy PLoS Pathogens 2012-01-05 [PMID: 22241988] (Immunohistochemistry, Human)
• Cattaruzza L, Gloghini A, Olivo K, Di Francia R, Lorenzon D, De Filippi R, Carbone A, Colombatti A, Pinto A, Aldinucci D Functional coexpression of Interleukin (IL)-7 and its receptor (IL-7R) on Hodgkin and Reed-Sternberg cells: Involvement of IL-7 in tumor cell growth and microenvironmental interactions of Hodgkin&#039;s lymphoma. Int. J. Cancer, 2009-09-01;125(5):1092-101. 2009-09-01 [PMID: 19391137] (Neutralization, Human)
• Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. AIDS Res. Hum. Retroviruses, 2008-03-01;24(3):453-62. 2008-03-01 [PMID: 18327985] (Luminex Development, Human)
• Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P Ultrasensitive flow-based immunoassays using single-molecule counting. Clin. Chem., 2007-09-21;53(11):1990-5. 2007-09-21 [PMID: 17890441] (ELISA Development, Human)
• Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Blood, 2007-02-08;109(10):4272-9. 2007-02-08 [PMID: 17289812] (Luminex Development, Human)
• Storek J, Gillespy T, Lu H, Joseph A, Dawson MA, Gough M, Morris J, Hackman RC, Horn PA, Sale GE, Andrews RG, Maloney DG, Kiem HP Interleukin-7 improves CD4 T-cell reconstitution after autologous CD34 cell transplantation in monkeys. Blood, 2003-01-23;101(10):4209-18. 2003-01-23 [PMID: 12543864] (Primate - Papio cynocephalus (Yellow Baboon))

Bioinformatics

• Gene Symbol: IL7
• Uniprot:
  • P13232()