IL-7 Antibody (7417.111)

Product Discontinued

IL-7 Antibody (7417.111) Summary

• Immunogen: Recombinant full length human IL7 protein.
• Localization: Secreted
• Specificity: The has the ability to neutralize the biological activity of recombinant human IL-7. It may also be used as a capture in a human IL-7 ELISA.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL7
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • ELISA 1-2 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: WB: Predicted molecular weight: 17 kDa. The exact concentration of antibody required to neutralize recombinant human IL7 activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity. The detection limit in immunoblotting for recombinant human IL7 is approximately 25 ng/lane and 300 ng/lane under nonreducing and reducing conditions, respectively. A working concentration of the capture antibody at 1-2 u g/ml (100ul/well) in combination with a biotinylated polyclonal detection antibody at 25-50 ng/ml (100ul/well) is recommended to detect human interleukin 7. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
• Control:

  IL-7 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS and 5% Trehalose
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for IL-7 Antibody (7417.111)

• IL7
• IL-7
• IL-7interleukin-7
• interleukin 7
• Lymphopoietin-1
• PBGF

Background

Interleukin-7 (IL-7, previously known as lymphopoietin-1 and pre-B-cell growth factor) is a hematopoietic growth factor that stimulates early B and T cells. It can also co-stimulate the proliferation of mature T cells with other factors such as ConA (concanavalin A) and IL-2. IL-7 can induce the formation of lymphokine-activated killer (LAK) cells, it also plays a role in the development of cytotoxic T lymphocytes (CTL). IL-7 can stimulate the production of pro-inflammatory cytokines and enhance the tumoricidal activity of monocytes/macrophages. It is produced by thymic stromal cells, spleen cells and keratinocytes. Human and murine IL-7 show cross-species reactivity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-7 Antibody (NB600-837)(2)

Publications using NB600-837

• Cai J, Zhao J, Liu D et al. Efficacy and Safety of Central Memory T Cells Combined With Adjuvant Therapy to Prevent Recurrence of Hepatocellular Carcinoma With Microvascular Invasion: A Pilot Study Frontiers in oncology 2021-12-03 [PMID: 34926296]
• Vandooren J, Pereira Rvs, Ugarte-Berzal E Et Al. Internal Disulfide Bonding and Glycosylation of Interleukin-7 Protect Against Proteolytic Inactivation by Neutrophil Metalloproteinases and Serine Proteases Frontiers in immunology 2021-06-30 [PMID: 34276694]

Bioinformatics

• Gene Symbol: IL7
• Entrez:
  • 3574(Human)