IL-6 Antibody - BSA Free

Western Blot: IL-6 Antibody [NBP2-78132] - Mouse IL-6 recombinant protein was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-IL-6 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.

Immunohistochemistry-Paraffin: IL-6 Antibody [NBP2-78132] - IHC analysis of a formalin fixed paraffin embedded tissue section of human appendix using 1:200 dilution of IL-6 antibody (NBP2-78132). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated membrane staining of glandular cells.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

IL-6 Antibody - BSA Free Summary

• Immunogen: Partial recombinant mouse IL-6 protein [UniProt P08505]
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IL6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1 - 2 ug/ml
• Application Notes: Western blot was performed with recombinant mouse IL-6 protein.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IL-6 Antibody - BSA Free

• B-cell differentiation factor
• B-cell stimulatory factor 2
• BSF2
• BSF-2
• CDF
• CTL differentiation factor
• HSF
• hybridoma growth factor
• IFNB2
• IFN-beta-2
• IL6
• IL-6
• Interferon beta-2
• interleukin 6 (interferon, beta 2)
• interleukin BSF-2
• interleukin-6
• MGI-2A

Background

IL-6 is a multifunctional cytokine that has an important role in host defense by regulating immune and inflammatory responses. It is expressed by a variety of normal and transformed cells including T cells, B cells, monocytes/macrophages, fibroblasts and various tumor cells. IL-6 has diverse biological functions, it stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. Recombinant human IL-6 is a 20.9 kDa protein containing 184 amino acid residues. Human IL-6 is active on murine cells but murine IL-6 is inactive on human cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for IL-6 Antibody (NBP2-78132). (Showing 1 - 1 of 1 FAQ).

1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
   • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

Bioinformatics

• Gene Symbol: IL6