IL-6 Antibody


Western Blot: IL-6 Antibody [NBP1-77894] - Shows detection of recombinant mouse IL-6 raised in E.coli. Recombinant truncated protein (0.1 ug, 21.7 kDa) was loaded on to an SDS-PAGE gel, and after separation, transferred more
Immunohistochemistry: IL-6 Antibody [NBP1-77894] - Shows nuclear positivity of islets of Langerhans (brown staining) and cytoplasmic staining in mouse pancreas at 10x and 20x (B & C). Staining was performed on Leica more

Product Details

Reactivity MuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
LYOPH mg/ml

Order Details

IL-6 Antibody Summary

This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with recombinant mouse IL-6 protein produced in E.coli (NP_112445.1).
Ion exchange chromatography
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
From 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
No Preservative
LYOPH mg/ml
Ion exchange chromatography
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.


Application Notes
Expect a band approximately 21.7 kDa in size corresponding - the mature 187 amino acid mouse IL-6 protein by western blotting in appropriate cell lysate or extract. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
24 kDa

Alternate Names for IL-6 Antibody

  • B cell stimulatory factor-2
  • B-cell differentiation factor
  • BSF-2
  • BSF2CTL differentiation factor
  • CDF
  • HGFHSFIFNB2Hybridoma growth factor
  • IFN-beta 2
  • IFN-beta-2
  • IL6
  • IL-6B-cell stimulatory factor 2
  • Interferon beta-2
  • interleukin 6 (interferon, beta 2)
  • interleukin BSF-2
  • interleukin-6
  • MGI-2A


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IL-6 Antibody (NBP1-77894). (Showing 1 - 1 of 1 FAQ).

  1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
    • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

Secondary Antibodies


Isotype Controls

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Contact Information

Product PDFs


Gene Symbol IL6

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