IL-5 Antibody (TRFK4) [Biotin]

405-ML) was serially diluted and captured by Rat Anti-Human/Mouse IL‑5 Monoclonal Antibody (Catalog # MAB405) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse IL‑5 Monoclonal Antibody (Catalog # MAB705) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" > 405-ML) was serially diluted and captured by Rat Anti-Human/Mouse IL‑5 Monoclonal Antibody (Catalog # MAB405) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse IL‑5 Monoclonal Antibody (Catalog # MAB705) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" title="Recombinant Mouse IL‑5 (Catalog # 405-ML) was serially diluted and captured by Rat Anti-Human/Mouse IL‑5 Monoclonal Antibody (Catalog # MAB405) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse IL‑5 Monoclonal Antibody (Catalog # MAB705) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" />

Recombinant Mouse IL‑5 (Catalog # <a class=

• Reactivity: Mu
• Applications: ELISA(Det)
• Clone: TRFK4
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Biotin
• Concentration: LYOPH

IL-5 Antibody (TRFK4) [Biotin] Summary

• Immunogen: Mouse T cell clone-derived partially purified recombinant mouse IL-5
• Specificity: Detects mouse IL-5 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human (rh) IL-5, recombinant mouse (rm) IL‑3, or rmGM-CSF is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: IL5
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Capture: Human/Mouse IL-5 Antibody (Catalog # MAB405)
  ELISA Detection: Mouse IL-5 Biotinylated Antibody (Catalog # BAM705)
  Standard: Recombinant Mouse IL-5 (Catalog # 405-ML)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-5 Antibody (TRFK4) [Biotin]

• BCDF mu
• B-cell differentiation factor I
• BCGFII
• EDF
• Eo-CSF
• Eosinophil differentiation factor
• IL5
• IL-5
• IL-5T-cell replacing factor
• interleukin 5 (colony-stimulating factor, eosinophil)
• interleukin-5
• TRF
• TRFB cell differentiation factor I

Background

IL-5 is a disulfide-linked homodimeric cytokine that is secreted by T cells. IL-5 promotes the proliferation, differentiation, and activation of eosinophils. It binds to a receptor complex consisting of one IL-5-specific alpha chain and one non-binding common beta chain that is shared with the receptors for GM-CSF and IL-3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-5 Antibody (BAM705)(6)

Publications using BAM705

• Yumiko Tanaka, Mai Yamagishi, Yasutaka Motomura, Takashi Kamatani, Yusuke Oguchi, Nobutake Suzuki et al. Time-dependent cell-state selection identifies transiently expressed genes regulating ILC2 activation Commun Biol 9/6/2023 [PMID: 37673922]
• W Dawicki, C Li, J Town, X Zhang, JR Gordon Therapeutic reversal of food allergen sensitivity by mature retinoic acid-differentiated dendritic cell induction of LAG3(+)CD49b(-)Foxp3(-) regulatory T�cells J. Allergy Clin. Immunol., 2016-10-26;139(5):1608-1620.e3. 2016-10-26 [PMID: 28277274] (ELISA Development (Detection), Mouse)
• Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine, 2012-01-25;58(2):169-77. 2012-01-25 [PMID: 22277799] (Luminex Development, Mouse)
• Wen H, Hogaboam CM, Gauldie J, Kunkel SL Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile. Am. J. Pathol., 2006-06-01;168(6):1940-50. 2006-06-01 [PMID: 16723709] (ELISA Development, Mouse)
• Nygaard UC, Ormstad H, Aase A, Lovik M The IgE adjuvant effect of particles: characterisation of the primary cellular response in the draining lymph node. Toxicology, 2005-01-15;206(2):181-93. 2005-01-15 [PMID: 15588912] (ELISA Development, Mouse)
• Lukacs NW, Berlin A, Schols D, Skerlj RT, Bridger GJ AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity. Am. J. Pathol., 2002-04-01;160(4):1353-60. 2002-04-01 [PMID: 11943720] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: IL5