IL-5 Antibodies

405-ML) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-5 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human/Mouse IL-5 Monoclonal Antibody (Catalog # MAB405). The ND₅₀ is typically 0.004-0.015 µg/mL." class="big_lightbox"> 405-ML) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-5 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human/Mouse IL-5 Monoclonal Antibody (Catalog # MAB405). The ND₅₀ is typically 0.004-0.015 µg/mL." alt="Recombinant Mouse IL-5 (405-ML) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-5 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human/Mouse IL-5 Monoclonal Antibody (Catalog # MAB405). The ND₅₀ is typically 0.004-0.015 µg/mL." class="big_thumb" />

205-IL) was serially diluted and captured by Rat Anti-Human/Mouse IL‑5 Monoclonal Antibody (Catalog # MAB405) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Human IL‑5 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_lightbox"> 205-IL) was serially diluted and captured by Rat Anti-Human/Mouse IL‑5 Monoclonal Antibody (Catalog # MAB405) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Human IL‑5 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_thumb" />

405-ML) was serially diluted and captured by Rat Anti-Human/Mouse IL‑5 Monoclonal Antibody (Catalog # MAB405) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse IL‑5 Monoclonal Antibody (Catalog # MAB705) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 405-ML) was serially diluted and captured by Rat Anti-Human/Mouse IL‑5 Monoclonal Antibody (Catalog # MAB405) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse IL‑5 Monoclonal Antibody (Catalog # MAB705) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> Fluorescent ICC Staining of Non-adherent Cells." alt="IL‑5 was detected in immersion fixed human peripheral blood lymphocytes using Mouse Anti-Human IL‑5 Monoclonal Antibody (Catalog # MAB605) at 5 µg/mL for 3 hours at room temperature. Cells were stained (red) and counterstained (green). Specific labeling was localized to the cytoplasm of PBMCs. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0103B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox"> MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0103B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />

2470-EL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Equine IL-5 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Equine IL-5 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF2470). The ND₅₀ is typically 0.500-6.00 µg/mL." class="big_lightbox"> 2470-EL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Equine IL-5 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Equine IL-5 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF2470). The ND₅₀ is typically 0.500-6.00 µg/mL." alt="Recombinant Equine IL-5 (2470-EL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Equine IL-5 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Equine IL-5 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF2470). The ND₅₀ is typically 0.500-6.00 µg/mL." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

205-IL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑5 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-205-NA). The ND₅₀ is typically 0.3-0.9 µg/mL." class="big_lightbox"> 205-IL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑5 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-205-NA). The ND₅₀ is typically 0.3-0.9 µg/mL." alt="Recombinant Human IL‑5 (Catalog # 205-IL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑5 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-205-NA). The ND₅₀ is typically 0.3-0.9 µg/mL." class="big_thumb" />

3137-PL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-5 (25 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Porcine IL-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3137). The ND₅₀ is typically <5 µg/mL." class="big_lightbox"> 3137-PL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-5 (25 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Porcine IL-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3137). The ND₅₀ is typically <5 µg/mL." alt="Recombinant Porcine IL-5 (Catalog # 3137-PL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-5 (25 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Porcine IL-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3137). The ND₅₀ is typically <5 µg/mL." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

545-RL) stimulates proliferation in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-5 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF545). The ND₅₀ is typically 2-6 µg/mL." class="big_lightbox"> 545-RL) stimulates proliferation in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-5 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF545). The ND₅₀ is typically 2-6 µg/mL." alt="Recombinant Rat IL-5 (Catalog # 545-RL) stimulates proliferation in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-5 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF545). The ND₅₀ is typically 2-6 µg/mL." class="big_thumb" />

205-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-5 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-5 Monoclonal Antibody (Catalog # MAB205). The ND₅₀ is typically 0.01-0.03 µg/mL." class="big_lightbox"> 205-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-5 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-5 Monoclonal Antibody (Catalog # MAB205). The ND₅₀ is typically 0.01-0.03 µg/mL." alt="Recombinant Human IL-5 (Catalog # 205-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-5 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-5 Monoclonal Antibody (Catalog # MAB205). The ND₅₀ is typically 0.01-0.03 µg/mL." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL-5 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA using Goat Anti-Canine IL-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1964) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

1964-CL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-5 (30 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Canine IL-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1964). The ND₅₀ is typically 0.75-3.0 µg/mL." class="big_lightbox"> 1964-CL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-5 (30 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Canine IL-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1964). The ND₅₀ is typically 0.75-3.0 µg/mL." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL‑5 was detected in immersion fixed feline peripheral blood mononuclear cells (PBMCs) using Goat Anti-Feline IL‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1795) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

1795-FL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL‑5 (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Feline IL‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1795). The ND₅₀ is typically 0.5-2.0 µg/mL." class="big_lightbox"> 1795-FL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL‑5 (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Feline IL‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1795). The ND₅₀ is typically 0.5-2.0 µg/mL." class="big_thumb" />

1795-FL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL-5 (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Feline IL-5 Monoclonal Antibody (Catalog # MAB1795). The ND₅₀ is typically 0.1-0.5 µg/mL." class="big_lightbox"> 1795-FL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL-5 (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Feline IL-5 Monoclonal Antibody (Catalog # MAB1795). The ND₅₀ is typically 0.1-0.5 µg/mL." alt="Recombinant Feline IL-5 (Catalog # 1795-FL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL-5 (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Feline IL-5 Monoclonal Antibody (Catalog # MAB1795). The ND₅₀ is typically 0.1-0.5 µg/mL." class="big_thumb" />

545-RL) stimulates proliferation in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-5 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat IL-5 Monoclonal Antibody (Catalog # MAB5452). The ND₅₀ is typically 0.2-1.0 µg/mL." class="big_lightbox"> 545-RL) stimulates proliferation in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-5 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat IL-5 Monoclonal Antibody (Catalog # MAB5452). The ND₅₀ is typically 0.2-1.0 µg/mL." alt="Recombinant Rat IL-5 (Catalog # 545-RL) stimulates proliferation in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-5 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat IL-5 Monoclonal Antibody (Catalog # MAB5452). The ND₅₀ is typically 0.2-1.0 µg/mL." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />