IL-5 Antibody (TRFK4)

Recombinant Mouse IL‑5 (Catalog # 405-ML) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑5 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑5 Monoclonal Antibody (Catalog # MAB705). The ND50 is typically 0.7‑3.5 µg/mL.

• Reactivity: Mu
• Applications: B/N
• Clone: TRFK4
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

IL-5 Antibody (TRFK4) Summary

• Immunogen: Mouse T cell clone-derived partially purified recombinant mouse IL-5
• Specificity: Detects mouse IL-5.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: IL5
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.7-3.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-5 Antibody (TRFK4)

• BCDF mu
• B-cell differentiation factor I
• BCGFII
• EDF
• Eo-CSF
• Eosinophil differentiation factor
• IL5
• IL-5
• IL-5T-cell replacing factor
• interleukin 5 (colony-stimulating factor, eosinophil)
• interleukin-5
• TRF
• TRFB cell differentiation factor I

Background

Interleukin 5 is a T cell-derived factor that promotes the proliferation, differentiation and activation of eosinophils. In mice, IL-5 has also been shown to be a growth and differentiation factor for B cells. Various names previously used to describe IL-5 include: T cell replacing factor (TRF), B cell growth factor II (BCGFII), B cell differentiation factor μ (BCDF μ), eosinophil differentiation factor (EDF) and eosinophil colony-stimulating factor (E o -CSF). Biologically active IL-5 is a disulfide-linked homodimer. The cDNAs for murine and human IL-5 encode precursor proteins with signal peptides that are cleaved to form mature proteins containing 113 and 115 amino acid residues, respectively. Murine and human IL-5 are 70% identical in their amino acid sequences and show species cross-reactivity. The genes for human and mouse IL-5 have been mapped to chromosome 5 and chromosome 11, respectively; closely linked to the genes for IL-3, IL-4 and GM-CSF. IL-5 exerts its activity on target cells by binding to specific cell surface receptors. The functional high-affinity receptor for human IL-5 has been shown to be composed of a low-affinity IL-5 binding alpha -subunit and a non-binding common beta -subunit that is shared with the high-affinity receptors for GM-CSF and IL-3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-5 Antibody (MAB705)(1)

Publication using MAB705

• Xavier-Elsas P, Santos-Maximiano E, Queto T, Mendonca-Sales S, Joseph D, Gaspar-Elsas MI, Vargaftig BB Ectopic lung transplantation induces the accumulation of eosinophil progenitors in the recipients&#039; lungs through an allergen- and interleukin-5-dependent mechanism. Clin. Exp. Allergy, 2007-01-01;37(1):29-38. 2007-01-01 [PMID: 17210039] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: IL5