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405-ML) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-5 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human/Mouse IL-5 Monoclonal Antibody (Catalog # MAB405). The ND50 is typically 0.004-0.015 µg/mL." class="big_lightbox" target="_blank">
205-IL) was serially diluted and captured by Rat Anti-Human/Mouse IL‑5 Monoclonal Antibody (Catalog # MAB405) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Human IL‑5 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_lightbox" target="_blank">
405-ML) was serially diluted and captured by Rat Anti-Human/Mouse IL‑5 Monoclonal Antibody (Catalog # MAB405) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse IL‑5 Monoclonal Antibody (Catalog # MAB705) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0103B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox" target="_blank">
IL-5 (T cell replacing factor (TRF), eosinophil differentiation factor (EDF)) is a hematopoietic growth factor that stimulates the proliferation, differentiation and activation of eosinophils. It is produced by mast cells, T cells and eosinophils and has an important role in activating cell-mediated immunity following parasitic infections. It belongs to the same cytokine family as IL-3 and GM-CSF. The biologically active IL-5 consists of an antiparallel homodimer linked by disulfide bonds. Human and murine IL-5 are 70% identical at the amino acid level and show species cross-reactivity. IL-5 exerts its effects on target cells by binding to specific cell surface receptors.
![Neutralization IL-5 Antibody (TRFK5) [Unconjugated]](http://images.novusbio.com/images/antibody/IL-5_MAB405_Block_Neutralize_9183.jpg)
![Immunocytochemistry IL-5 Antibody (9906) [Unconjugated]](http://images.novusbio.com/images/antibody/IL-5_MAB605_Immunocytochemistry_7379.jpg)
![Sandwich ELISA IL-5 Antibody Pair [HRP]](http://images.novusbio.com/fullsize/IL-5-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79517-img0001.jpg)
![N/A IL-5 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_IL5_M5000_ELISA_935.jpg)
![N/A IL-5 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_IL5_DY405_ELISA_2002.jpg)
![N/A IL-5 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_IL5_DY205_ELISA_1756.jpg)
![Bioactivity IL-5 [Unconjugated]](http://images.novusbio.com/fullsize/protein/IL-5_205-IL_725.jpg)
![ELISA IL-5 Antibody (TRFK5) [Unconjugated]](http://images.novusbio.com/images/mab405_human-mouse-il-5-mab-clone-trfk5-elisa-639201775911895873.jpg)
![ELISA IL-5 Antibody (TRFK5) [Unconjugated]](http://images.novusbio.com/images/mab405_human-mouse-il-5-mab-clone-trfk5-elisa-639201778316376459.jpg)
![Intracellular Staining by Flow Cytometry IL-5 Antibody (9906) [Unconjugated]](http://images.novusbio.com/images/antibody/IL-5_MAB605_Flow_Cytometry_8546.jpg)
![Immunohistochemistry IL-5 Antibody (9906) [Unconjugated]](http://images.novusbio.com/fullsize/antibody/mab605_human-il-5-mab-clone-9906-immunohistochemistry-1772025134211..jpg)
![N/A IL-5 [HRP]](http://images.novusbio.com/fullsize/elisa/IL-5_M5000_ELISA_237.jpg)
![SDS-Page IL-5 [Unconjugated]](http://images.novusbio.com/fullsize/protein/IL-5_205-IL_723.jpg)
