Human IL-4 ELISA Kit (Colorimetric)

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Human IL-4 ELISA Kit [NBP1-91171] - Data for Human IL-4 Platinum ELISA

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma
Standard Curve Range
7.8 - 500 pg/mL
Sensitivity
1.3 pg/mL

Order Details

Human IL-4 ELISA Kit (Colorimetric) Summary

Specificity
The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-4 positive serum. There was no crossreactivity detected.
Standard Curve Range
7.8 - 500 pg/mL
Sensitivity
1.3 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
5.60%
Intra-Assay
4.80%
Spike Recovery
The unspiked serum was used as blank in these experiments. The recovery ranged from 75% to 111% with an overall mean recovery of 94%.
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IL4

Applications/Dilutions

Application Notes
This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  2. Streptavidin-HRP
  3. Substrate Solution
  4. Stop Solution (1M Phosphoric acid)
  5. Sample Diluent
  6. Biotin-Conjugate anti-human IL-4 polyclonal antibody
  7. Human IL-4 Standard lyophilized, 1 ng/mL upon reconstitution
  8. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  9. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-4
  10. Amplification Diluent Concentrate (2x)
  11. Amplification Reagent II
  12. Amplification Reagent I*
  13. Adhesive Films
  14. * reagent contains ethyl alcohol

Alternate Names for Human IL-4 ELISA Kit (Colorimetric)

  • B cell growth factor 1
  • BCDF
  • B-cell stimulatory factor 1
  • BCGF1
  • BCGF-1
  • binetrakin
  • BSF1
  • BSF-1
  • IL4
  • IL-4
  • IL-4B_cell stimulatory factor 1
  • interleukin 4
  • interleukin-4
  • Lymphocyte stimulatory factor 1
  • MGC79402
  • pitrakinra

Background

Human IL-4 exists in molecular weight forms between 15,000 and 19,000 daltons, representing variable glycosylation. The gene for human IL-4 is found on the long arm of chromsome 5 in close association with genes for IL-13, IL-5 GM-CSF and IL-3. IL-4 mediates its function by binding to receptors expressed on target cells. The IL-4 receptors exhibit an affinity of approximately 10-10 M. Receptors exist on freshly prepared B and T lymphocytes and macrophages, as well as on various cell lines including lymphoid cells, mast cell lines, a variety of other hematopoietic cell lines, fibroblasts and stromal cell lines. On T and B cells, receptors are present in low numbers (appr. 400), which are reported to be up-regulated by IL-2 and IL-4. IL-4 is produced by a particular subset of T helper cells, the TH2 cells. These cells tend to make a specific set of lymphokines including IL-4, IL-5, IL-6, IL-10, IL-13, IL-3 and GM-CSF and fail to produce IL-2, IFN-gamma, and lymphotoxin (TNF-beta). Apart from T cells, it has been shown that mast cells can produce IL-4. IL-4 exerts numerous effects on various hematopoietic cell types. On B cells, IL-4 promotes immunological class switching to IgE and IgG1 isotypes and upregulates MHC class II and CD23 expression. It can promote survival, growth, and differentiation of both T and B lymphocytes, mast cells, and endothelial cells. In addition, IL-4 can inhibit the production of TNF, IL-1, and IL-6 by macrophages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol IL4
Entrez