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Product Details

Reactivity HuSpecies Glossary
Applications ELISA
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Suitable Sample Type
Cell culture supernatant, serum, plasma

Order Details

IL-4 ELISA Kit Summary

Standard Curve Range
7.8 - 500 pg/mL
1.3 pg/mL
Kit Type

Packaging, Storage & Formulations

Storage of components varies. See protocol for specific instructions.
Concentration is not relevant for this product. Please see the protocols for proper use of this product.


Application Notes
The human IL-4 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-4

Kit Components

  1. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-4
  2. Biotin-Conjugate anti-human IL-4 monoclonal antibody
  3. Streptavidin-HRP
  4. Human IL-4 Standard lyophilized, 1 ng/mL upon reconstitution
  5. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  6. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  7. Substrate Solution (tetramethyl-benzidine)
  8. Stop Solution (1M Phosphoric acid)
  9. Adhesive Films

Reactivity Notes



Colored dyes for optional use with this kit are available and will be provided free of charge upon request. Please refer to catalog number NBP2-41404.

Alternate Names for IL-4 ELISA Kit

  • B cell growth factor 1
  • BCDF
  • B-cell stimulatory factor 1
  • BCGF1
  • BCGF-1
  • binetrakin
  • BSF1
  • BSF-1
  • IL4
  • IL-4B_cell stimulatory factor 1
  • interleukin 4
  • interleukin-4
  • Lymphocyte stimulatory factor 1
  • MGC79402
  • pitrakinra


Human IL-4 exists in molecular weight forms between 15,000 and 19,000 daltons, representing variable glycosylation. The gene for human IL-4 is found on the long arm of chromsome 5 in close association with genes for IL-13, IL-5 GM-CSF and IL-3. IL-4 mediates its function by binding to receptors expressed on target cells. The IL-4 receptors exhibit an affinity of approximately 10-10 M. Receptors exist on freshly prepared B and T lymphocytes and macrophages, as well as on various cell lines including lymphoid cells, mast cell lines, a variety of other hematopoietic cell lines, fibroblasts and stromal cell lines. On T and B cells, receptors are present in low numbers (appr. 400), which are reported to be up-regulated by IL-2 and IL-4. IL-4 is produced by a particular subset of T helper cells, the TH2 cells. These cells tend to make a specific set of lymphokines including IL-4, IL-5, IL-6, IL-10, IL-13, IL-3 and GM-CSF and fail to produce IL-2, IFN-gamma, and lymphotoxin (TNF-beta). Apart from T cells, it has been shown that mast cells can produce IL-4. IL-4 exerts numerous effects on various hematopoietic cell types. On B cells, IL-4 promotes immunological class switching to IgE and IgG1 isotypes and upregulates MHC class II and CD23 expression. It can promote survival, growth, and differentiation of both T and B lymphocytes, mast cells, and endothelial cells. In addition, IL-4 can inhibit the production of TNF, IL-1, and IL-6 by macrophages.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Contact Information

Product PDFs


Gene Symbol IL4

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