Human IL-4 ELISA Kit (Colorimetric) Summary
The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-4 positive serum. There was no crossreactivity detected.
|Standard Curve Range
7.8 - 500 pg/mL
The unspiked serum was used as blank in these experiments.
The recovery ranged from 75% to 111% with an overall mean recovery of 94%.
ELISA Kit (Colorimetric)
This kit is an enzyme-linked immunosorbent assay for quantitative detection.
Packaging, Storage & Formulations
Storage of components varies. See protocol for specific instructions.
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
- Substrate Solution
- Human IL-4 Standard lyophilized, 1 ng/mL upon reconstitution
- Stop Solution (1M Phosphoric acid)
- Sample Diluent
- Biotin-Conjugate anti-human IL-4 polyclonal antibody
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-4
- Amplification Reagent I*
- Amplification Reagent II
- Amplification Diluent Concentrate (2x)
- Adhesive Films
- * reagent contains ethyl alcohol
Alternate Names for Human IL-4 ELISA Kit (Colorimetric)
- B cell growth factor 1
- B-cell stimulatory factor 1
- IL-4B_cell stimulatory factor 1
- interleukin 4
- Lymphocyte stimulatory factor 1
Human IL-4 exists in molecular weight forms between 15,000 and 19,000 daltons, representing variable glycosylation. The gene for human IL-4 is found on the long arm of chromsome 5 in close association with genes for IL-13, IL-5 GM-CSF and IL-3. IL-4 mediates its function by binding to receptors expressed on target cells. The IL-4 receptors exhibit an affinity of approximately 10-10 M. Receptors exist on freshly prepared B and T lymphocytes and macrophages, as well as on various cell lines including lymphoid cells, mast cell lines, a variety of other hematopoietic cell lines, fibroblasts and stromal cell lines. On T and B cells, receptors are present in low numbers (appr. 400), which are reported to be up-regulated by IL-2 and IL-4. IL-4 is produced by a particular subset of T helper cells, the TH2 cells. These cells tend to make a specific set of lymphokines including IL-4, IL-5, IL-6, IL-10, IL-13, IL-3 and GM-CSF and fail to produce IL-2, IFN-gamma, and lymphotoxin (TNF-beta). Apart from T cells, it has been shown that mast cells can produce IL-4. IL-4 exerts numerous effects on various hematopoietic cell types. On B cells, IL-4 promotes immunological class switching to IgE and IgG1 isotypes and upregulates MHC class II and CD23 expression. It can promote survival, growth, and differentiation of both T and B lymphocytes, mast cells, and endothelial cells. In addition, IL-4 can inhibit the production of TNF, IL-1, and IL-6 by macrophages.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed
for 6 months from date of receipt.
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