IL-4 Antibody [Unconjugated]

Recombinant Canine IL‑4 (Catalog # 754-CL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL‑4 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF754). The ND50 is typically 2-8 µg/mL.

• Reactivity: Ca
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-4 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant canine IL-4
  His25-His132
  Accession # O77762
• Specificity: Detects canine IL-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human IL-4 is observed, 15% cross-reactivity with recombinant feline IL-4 and recombinant porcine IL-4 is observed and less than 1% cross-reactivity with recombinant cotton rat IL-4, recombinant mouse IL-4, and recombinant rat IL-4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL4
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 2-8 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-4 Antibody [Unconjugated]

• B cell growth factor 1
• BCDF
• B-cell stimulatory factor 1
• BCGF1
• BCGF-1
• binetrakin
• BSF1
• BSF-1
• IL4
• IL-4
• IL-4B_cell stimulatory factor 1
• IL4E12
• interleukin 4
• interleukin-4
• Lymphocyte stimulatory factor 1
• MGC79402
• pitrakinra

Background

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa‑18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (5). Canine IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature canine IL-4 shares 81%, 61%, 46%, 38%, and 37% aa sequence identity with feline, bovine, human, mouse, and rat IL-4, respectively. IL-4 exerts its effects through two receptor complexes (6, 7). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on non‑hematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4⁺ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgE in B cells, acquisition of the Th2 phenotype by naïve CD4⁺ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (8‑11). IL-4 plays a dominant role in the development of allergic inflammation and asthma (10, 12).

1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
3. Khatlani, T. et al. (1999) J. Vet. Med. Sci. 61:967.
4. van der Kaaij, S.Y. et al. (1999) Immunogenetics 49:142.
5. Redfield, C. et al. (1991) Biochemistry 30:11029.
6. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
7. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
8. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
9. Corthay, A. (2006) Scand. J. Immunol. 64:93.
10. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
11. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
12. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-4 Antibody (AF754)(1)

Publication using AF754

• Rai MF, Graeve T, Twardziok S, Schmidt MF Evidence for regulated interleukin-4 expression in chondrocyte-scaffolds under in vitro inflammatory conditions. PLoS ONE, 2011-10-03;6(10):e25749. 2011-10-03 [PMID: 21991344] (Neutralization, Canine)

Bioinformatics

• Gene Symbol: IL4
• Uniprot:
  • O77762()