IL-4 Antibody

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Recombinant Human IL‑4 (Catalog # 204-IL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑4 (0.5 ng/mL) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Neut
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

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Catalog# & Conjugate Size Price

IL-4 Antibody Summary

Immunogen
E. coli-derived recombinant human IL‑4
His25-Ser153
Accession # NP_000580
Specificity
Detects human IL-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant feline IL-4, recombinant canine IL-4, recombinant equine IL-4, and recombinant porcine IL-4 is observed and less than 1% cross-reactivity with recombinant mouse IL-4 and recombinant rat IL-4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL4
Purity
Immunogen affinity purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Neutralization 0.02-0.1 ug/mL
Publications
Read Publications using
AF-204-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-4 Antibody

  • B cell growth factor 1
  • BCDF
  • B-cell stimulatory factor 1
  • BCGF1
  • BCGF-1
  • binetrakin
  • BSF1
  • BSF-1
  • IL4
  • IL-4
  • IL-4B_cell stimulatory factor 1
  • interleukin 4
  • interleukin-4
  • Lymphocyte stimulatory factor 1
  • MGC79402
  • pitrakinra

Background

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses. It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure. Human IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Alternate splicing generates an isoform with a 16 aa internal deletion. Mature human IL-4 shares 55%, 39%, and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities. IL-4 exerts its effects through two receptor complexes. The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma  chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Neut

Publications for IL-4 Antibody (AF-204-NA)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: IHC, IHC - Not specified, Neut.


Filter By Application
IHC
(1)
IHC - Not specified
(1)
Neut
(5)
All Applications
Filter By Species
Human
(7)
All Species
Showing Publications 1 - 7 of 7.
Publications using AF-204-NA Applications Species
Guo PF, Du MR, Wu HX, Lin Y, Jin LP, Li DJ Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans. Blood, 2010;116(12):2061-9. 2010 [PMID: 20538796] (IHC - Not specified,, Human) IHC - Not specified Human
Heo YJ, Joo YB, Oh HJ, Park MK, Heo YM, Cho ML, Kwok SK, Ju JH, Park KS, Cho SG, Park SH, Kim HY, Min JK IL-10 suppresses Th17 cells and promotes regulatory T cells in the CD4+ T cell population of rheumatoid arthritis patients. Immunol. Lett., 2010;127(2):150-6. 2010 [PMID: 19895848] (Neut,, Human) Neut Human
Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells. Eur. J. Immunol., 2008;38(4):1012-23. 2008 [PMID: 18350544] (Neut,, Human) Neut Human
Withers DR, Fiorini C, Fischer RT, Ettinger R, Lipsky PE, Grammer AC T cell-dependent survival of CD20+ and CD20- plasma cells in human secondary lymphoid tissue. Blood, 2007;109(11):4856-64. 2007 [PMID: 17299094] (Neut,, Human) Neut Human
Methe H, Wiegand D, Welsch U, Meiser B, Nabauer M, Koglin J Peripheral expansion of circulating T-helper 1 cells predicts coronary endothelial dysfunction after cardiac transplantation. J. Heart Lung Transplant., 2005;24(7):833-40. 2005 [PMID: 15982610] (IHC,, Human) IHC Human
Birebent B, Lorho R, Lechartier H, de Guibert S, Alizadeh M, Vu N, Beauplet A, Robillard N, Semana G Suppressive properties of human CD4+CD25+ regulatory T cells are dependent on CTLA-4 expression. Eur. J. Immunol., 2004;34(12):3485-96. 2004 [PMID: 15484187] (Neut,, Human) Neut Human
Hakonarson H, Whelan R, Leiter J, Kim C, Chen M, Campbell D, Grunstein MM T lymphocyte-mediated changes in airway smooth muscle responsiveness are attributed to induced autocrine release and actions of IL-5 and IL-1beta. J. Allergy Clin. Immunol., 2002;110(4):624-33. 2002 [PMID: 12373272] (Neut,, Human) Neut Human

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Bioinformatics

Gene Symbol IL4
Entrez
Uniprot