IL-4 Antibody (3010) (MAB604)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA(Cap)
Clone
3010
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

IL-4 Antibody (3010) Summary

Immunogen
E. coli-derived recombinant human IL‑4
His25-Ser153
Accession # P05112.1
Specificity
Detects human IL-4 in ELISAs and Western blots. In sandwich ELISAs, no cross-reactivity or interference was observed with recombinant human (rh) IL‑1 alpha, rhIL‑1 beta, rhIL‑2, rhIL-3, rhIL-6, rhIL-7, rhIL-8, rhG-CSF, rhGM-CSF, rhTNF-alpha, rhTNF‑ beta, rhLIF, rhTGF‑ beta 1, recombinant mouse (rm) IL‑1 beta, rmIL-6, rmIL-7, rmGM-CSF, bovine FGF acidic, bovine FGF basic, porcine PDGF, human TGF‑ beta 1, porcine TGF‑ beta 1.2, and porcine TGF‑ beta 2.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL4
Purity
Protein A or G purified from ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Detection: Human IL-4 Biotinylated Antibody (Catalog number BAF204)
Standard: Recombinant Human IL-4 (Catalog number 204-IL)
Publications
Read Publications using
MAB604 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Protein A or G purified from ascites
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-4 Antibody (3010)

  • B cell growth factor 1
  • BCDF
  • B-cell stimulatory factor 1
  • BCGF1
  • BCGF-1
  • binetrakin
  • BSF1
  • BSF-1
  • IL4
  • IL-4
  • IL-4B_cell stimulatory factor 1
  • interleukin 4
  • interleukin-4
  • Lymphocyte stimulatory factor 1
  • MGC79402
  • pitrakinra

Background

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1-3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Human IL-4 is synthesized with a 24 aa signal sequence. Alternate splicing generates an isoform with a 16 aa internal deletion. Mature human IL-4 shares 55%, 39% and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5-7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma  chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10-13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-4 Antibody (MAB604)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: Array Development, ELISA Development, Electron Microscopy, Flow, Luminex Development.


Filter By Application
Array Development
(1)
ELISA Development
(3)
Electron Microscopy
(1)
Flow
(1)
Luminex Development
(3)
All Applications
Filter By Species
Human
(9)
All Species
Showing Publications 1 - 9 of 9.
Publications using MAB604 Applications Species
Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. AIDS Res. Hum. Retroviruses, 2008;24(3):453-62. 2008 [PMID: 18327985] (Luminex Development,, Human) Luminex Development Human
Liotta F, Angeli R, Cosmi L, Fili L, Manuelli C, Frosali F, Mazzinghi B, Maggi L, Pasini A, Lisi V, Santarlasci V, Consoloni L, Angelotti ML, Romagnani P, Parronchi P, Krampera M, Maggi E, Romagnani S, Annunziato F Toll-like receptors 3 and 4 are expressed by human bone marrow-derived mesenchymal stem cells and can inhibit their T-cell modulatory activity by impairing Notch signaling. Stem Cells, 2007;26(1):279-89. 2007 [PMID: 17962701] (ELISA Development,, Human) ELISA Development Human
Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1. AIDS, 2007;21(10):1263-72. 2007 [PMID: 17545702] (Luminex Development,, Human) Luminex Development Human
Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Blood, 2007;109(10):4272-9. 2007 [PMID: 17289812] (Luminex Development,, Human) Luminex Development Human
Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. Clin. Immunol., 2006;120(2):220-31. 2006 [PMID: 16782408] (ELISA Development,, Human) ELISA Development Human
Spencer LA, Melo RC, Perez SA, Bafford SP, Dvorak AM, Weller PF Cytokine receptor-mediated trafficking of preformed IL-4 in eosinophils identifies an innate immune mechanism of cytokine secretion. Proc. Natl. Acad. Sci. U.S.A., 2006;103(9):3333-8. 2006 [PMID: 16492782] (Electron Microscopy,, Human) Electron Microscopy Human
Pavon L, Kiryushko D, Novitskaya V, Sandoval-Lopez G, Eugenia Hernandez M, Soroka V, Klingelhofer J, Loria F, Estrada I, Lukanidin E, Berezin V, Perez M, Moreno J, Bock E, Avila U, Leff P, Anton B, Heinze G Th2 cytokine response in Major Depressive Disorder patients before treatment. J. Neuroimmunol., 2006;172(1):156-65. 2006 [PMID: 16457895] (ELISA Development,, Human) ELISA Development Human
Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells. Immunology, 2004;112(2):274-89. 2004 [PMID: 15147571] (Flow,, Human) Flow Human
Copeland S, Siddiqui J, Remick D Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines. J. Immunol. Methods, 2004;284(1):99-106. 2004 [PMID: 14736420] (Array Development,, Human) Array Development Human

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Bioinformatics

Gene Symbol IL4
Entrez
Uniprot