IL-3R alpha/CD123 Overexpression Lysate

Images

 
Western Blot: IL3RA Overexpression Lysate (Adult Normal) [NBL1-11956] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IL3RA.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

IL-3R alpha/CD123 Overexpression Lysate Summary

Description

IL-3R alpha/CD123 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC206420

Accession#: NM_002183

Protein Tag: C-MYC/DDK
Gene
IL3RA

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
41.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for IL-3R alpha/CD123 Overexpression Lysate

  • CD123 antigen
  • CD123
  • hIL3Ra
  • hIL-3Ra
  • IL-3 R alpha
  • IL-3 receptor subunit alpha
  • IL3R alpha
  • IL-3R alpha
  • IL-3R subunit alpha
  • IL3R
  • IL3RA
  • IL-3Ra
  • IL-3R-alpha
  • IL3RAY
  • IL3RX
  • IL3RY
  • interleukin 3 receptor, alpha (low affinity)
  • interleukin-3 receptor subunit alpha
  • MGC34174

Background

The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-72042
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, WB
NB110-97871
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
7268-CT
Species: Hu
Applications: BA
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
203-IL/CF
Species: Hu
Applications: BA
NBP1-19371
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
NB600-1071
Species: Hu(-), Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-15184
Species: Hu, Rt
Applications: Flow-CS, Flow, ICC/IF, IHC, IHC-P, Simple Western
AF1376
Species: Hu
Applications: CyTOF-ready, Flow, IHC, WB
1137-SL
Species: Hu
Applications: BA
NBP2-93743
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
DC140
Species: Hu
Applications: ELISA
MAB1774
Species: Hu
Applications: CyTOF-ready, Flow, ICC
NBP2-25208
Species: Hu, Mu, Rt
Applications: Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P, KO, WB
6507-IL/CF
Species: Hu
Applications: BA
AF1356
Species: Hu, Mu
Applications: CyTOF-ready, Flow, IHC, Simple Western, WB
NB100-683
Species: Hu, Mu, Rt
Applications: Dual ISH-IHC, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB

Publications for IL-3R alpha/CD123 Lysate (NBL1-11956) (0)

There are no publications for IL-3R alpha/CD123 Lysate (NBL1-11956).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for IL-3R alpha/CD123 Lysate (NBL1-11956) (0)

There are no reviews for IL-3R alpha/CD123 Lysate (NBL1-11956). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IL-3R alpha/CD123 Lysate (NBL1-11956) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IL-3R alpha/CD123 Products

Bioinformatics Tool for IL-3R alpha/CD123 Lysate (NBL1-11956)

Discover related pathways, diseases and genes to IL-3R alpha/CD123 Lysate (NBL1-11956). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IL-3R alpha/CD123 Lysate (NBL1-11956)

Discover more about diseases related to IL-3R alpha/CD123 Lysate (NBL1-11956).
 

Pathways for IL-3R alpha/CD123 Lysate (NBL1-11956)

View related products by pathway.

PTMs for IL-3R alpha/CD123 Lysate (NBL1-11956)

Learn more about PTMs related to IL-3R alpha/CD123 Lysate (NBL1-11956).
 

Research Areas for IL-3R alpha/CD123 Lysate (NBL1-11956)

Find related products by research area.

Blogs on IL-3R alpha/CD123

There are no specific blogs for IL-3R alpha/CD123, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our IL-3R alpha/CD123 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol IL3RA