IL-3R alpha/CD123 Overexpression Lysate


Western Blot: IL3RA Overexpression Lysate (Adult Normal) [NBL1-11956] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IL3RA.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

IL-3R alpha/CD123 Overexpression Lysate Summary


IL-3R alpha/CD123 Transient Overexpression Lysate

Expression Host: HEK293T

Plasmid: RC206420

Accession#: NM_002183

Protein Tag: C-MYC/DDK


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
41.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for Array



HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for IL-3R alpha/CD123 Overexpression Lysate

  • CD123 antigen
  • CD123
  • hIL3Ra
  • hIL-3Ra
  • IL-3 R alpha
  • IL-3 receptor subunit alpha
  • IL3R alpha
  • IL-3R alpha
  • IL-3R subunit alpha
  • IL3R
  • IL3RA
  • IL-3Ra
  • IL-3R-alpha
  • IL3RAY
  • IL3RX
  • IL3RY
  • interleukin 3 receptor, alpha (low affinity)
  • interleukin-3 receptor subunit alpha
  • MGC34174


The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for IL-3R alpha/CD123 Lysate (NBL1-11956) (0)

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Gene Symbol IL3RA