IL-3R alpha/CD123 Antibody (6H6) [PE/Cy7]

Flow Cytometry: IL-3 R alpha/CD123 Antibody (6H6) [PE/Cy7] [NBP2-00467] - Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE-Cy7 (NBP1-42944) (open histogram) or Anti-Human CD123 PE-Cy7 (filled histogram). Cells in the lymphocyte gate were used for analysis.

Flow Cytometry: IL-3 R alpha/CD123 Antibody (6H6) [PE/Cy7] [NBP2-00467] - Human buffy coat. Image verfied by customer.

Product Discontinued

IL-3R alpha/CD123 Antibody (6H6) [PE/Cy7] Summary

• Immunogen: Tissue / cell preparation. IL3R transfected COS cells.
• Localization: Type I membrane protein.
• Specificity: IL3RA (6H6)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL3RA
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
• Application Notes: Flow Cytometry Use 1ug for 10^6 cells in 100 ul volume or 100 ul of whole blood. This antibody has been tested by tested by immunofluorescent staining with flow cytometric analysis. IHC-Fr: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects a band of approximately 70 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. Although not tested this antibody may be useful in IHC-P and Immunoprecipitation.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS (pH 7.2) with 0.1% gelatin and 0.2% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 5 mg/ml
• Purity: Protein A or G purified

Alternate Names for IL-3R alpha/CD123 Antibody (6H6) [PE/Cy7]

• CD123 antigen
• CD123
• hIL3Ra
• hIL-3Ra
• IL-3 R alpha
• IL-3 receptor subunit alpha
• IL3R alpha
• IL-3R alpha
• IL-3R subunit alpha
• IL3R
• IL3RA
• IL-3Ra
• IL-3R-alpha
• IL3RAY
• IL3RX
• IL3RY
• interleukin 3 receptor, alpha (low affinity)
• interleukin-3 receptor subunit alpha
• MGC34174

Background

The 6H6 monoclonal antibody reacts with human CD123, the a chain of the IL-3 receptor. This 60-70 kDa transmembrane protein binds to IL-3 with low affinity by itself, and when associated with CD131 (common beta chain) binds IL-3 with high affinity. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, and megakaryocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for IL-3R alpha/CD123 Antibody (NBP2-00467) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Flow Human 12/15/2014

Summary

• Application: Flow Cytometry
• Sample Tested: human buffy coat
• Species: Human

Bioinformatics

• Gene Symbol: IL3RA