IL-38/IL-1F10 Antibody (798036) [Unconjugated]

Western blot shows lysates of mouse skin tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse IL-38/IL-1F10 Monoclonal Antibody (Catalog # MAB7774) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-38/IL-1F10 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse IL-38/IL-1F10 Monoclonal Antibody (Catalog # MAB7774, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Mu
• Applications: WB, CyTOF-ready, Flow
• Clone: 798036
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-38/IL-1F10 Antibody (798036) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse IL-38/IL-1F10
  Met1-Arg152
  Accession # Q8R459
• Specificity: Detects mouse IL-38/IL-1F10 in ELISA and Western Blot.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: IL1F10
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-38/IL-1F10 Antibody (798036) [Unconjugated]

• FIL1- theta
• FKSG75
• IL1F10
• IL-1HY2
• IL38
• IL-38
• interleukin 1 family, member 10 (theta)
• UNQ6119/PRO20041

Background

IL-1F10, also known as IL-1HY2, is a secreted protein that shares sequence and structural similarity with IL-1 receptor antagonist and other IL-1 family members. It is expressed in basal epithelia of the skin and in proliferating germinal center CD20⁺ B cells. IL-1F10 binds soluble IL-1RI in vitro and may participate in regulation of immune responses. Mouse IL-1F10 shares 82% and 93% aa identity with human and rat IL-1F10, respectively. IL-1F10 is predicted to be 17 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-38/IL-1F10 Antibody (MAB7774)(1)

Publication using MAB7774

• Y Ge, J Chen, Y Hu, X Chen, M Huang IL-38 Alleviates Inflammation in Sepsis in Mice by Inhibiting Macrophage Apoptosis and Activation of the NLRP3 Inflammasome Mediators of Inflammation, 2021-12-17;2021(0):6370911. 2021-12-17 [PMID: 34955683] (Neutralization, Western Blot, Mouse)

Bioinformatics

• Gene Symbol: IL1F10
• Uniprot:
  • Q8R459()