Plastin L Antibody

Western Blot: Plastin L Antibody [NBP2-19860] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with L-Plastin antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Plastin L Antibody [NBP2-19860] - Plastin L antibody detects Plastin L protein by immunofluorescent analysis. Sample: A549 cells were fixed in ice-cold MeOH for 5 min. Green: Plastin Lstained by Plastin L antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.

Immunohistochemistry-Paraffin: Plastin L Antibody [NBP2-19860] - Paraffin-embedded mouse thymus gland. Plastin L antibody diluted at 1:500.

Western Blot: Plastin L Antibody [NBP2-19860] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with L-Plastin antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: Plastin L Antibody [NBP2-19860] - arious whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with L-Plastin antibody diluted at 1:10000.

Western Blot: Plastin L Antibody [NBP2-19860] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with L-Plastin antibody diluted at 1:10000.

Western Blot: Plastin L Antibody [NBP2-19860] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Plastin L antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Plastin L Antibody [NBP2-19860] - A431 cells were fixed in ice-cold MeOH for 5 min. Green: L-Plastin protein stained by L-Plastin antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Plastin L Antibody [NBP2-19860] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Plasitn L antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Bv, Ch, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Plastin L Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Plastin L. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Rat (97%), Chicken (92%), Rhesus Macaque (99%), Bovine (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LCP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:5000-1:20000
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (86%), Xenopus laevis (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for Plastin L Antibody

• CP64
• DKFZp781A23186
• FLJ25423
• FLJ26114
• FLJ39956
• LC64PPLS2bA139H14.1 (lymphocyte cytosolic protein 1 (L-plastin))
• LCP-1
• LPL
• L-plastin (Lymphocyte cytosolic protein 1) (LCP-1) (LC64P)
• L-PLASTIN
• lymphocyte cytosolic protein 1 (L-plastin)
• Lymphocyte cytosolic protein 1
• Lymphocyte cytosolic protein-1 (plasmin)
• plastin 2
• plastin-2

Background

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). However, L-plastin has been found in many types of malignant human cells of non-hemopoietic origin suggesting that its expression is induced accompanying tumorigenesis in solid tissues. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LCP1
• Uniprot:
  • P13796(Human)