IL-33 Antibody - BSA Free

Western Blot: IL-33 Antibody [NBP2-24858] - Analysis of IL-33 using IL-33 polyclonal antibody. HUVEC cell lysate probed with IL-33 antibody at 1 ug/ml in the 1) absence and 2) presence of immunizing peptide.

Simple Western: IL-33 Antibody [NBP2-24858] - Lane view shows a specific band for IL-33 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Ca, Pm, Pm
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

IL-33 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit IL-33 Antibody - BSA Free (NBP2-24858) is a polyclonal antibody validated for use in WB and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A portion of amino acids 50-100 of human IL-33 was used as the immunogen for this antibody.
• Specificity: 100% homologous in human (isoforms CRA_a and CRA_b).
• Predicted Species: Primate (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IL33
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Simple Western 1:200
  • Western Blot 0.5-2 ug/ml
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in Jurkat lysate 0.05 mg/mL, separated by Size, antibody dilution of 1:200, apparent MW was 60 kDa.

Reactivity Notes

Chimpanzee (91%), Bovine (85%), Mouse (78%).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 0.2% Gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IL-33 Antibody - BSA Free

• C9orf26
• C9orf26chromosome 9 open reading frame 26 (NF-HEV)
• DKFZp586H0523
• DVS27
• DVS27-related protein
• IL1F11
• IL-1F11
• IL33
• IL-33
• interleukin 33
• Interleukin-1 family member 11
• interleukin-33
• NFHEV
• NF-HEV
• NF-HEVNFEHEV
• Nuclear factor from high endothelial venules
• RP11-575C20.2

Background

Interleukin 33 (IL-33) is a 32kDa proinflammatory cytokine and intracellular nuclear factor with transcriptional regulatory properties. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12kDa and C-terminal fragment of 18kDa by cleavage of caspase-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL33
• Uniprot:
  • O95760()