IL-33 Antibody [Biotin]

bEND.3 cells were stained with Goat Anti-Mouse IL‑33 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3626, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram) followed by Streptavidin-Allophycocyanin (Catalog # F0050). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Mu
• Applications: WB, ELISA(Det), Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

IL-33 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant mouse IL-33
  Ser109-Ile266
  Accession # Q8BVZ5
• Specificity: Detects mouse IL-33 in ELISAs and Western blots. In sandwich ELISAs, less than 2% cross-reactivity with recombinant human IL‑33 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL33
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse IL-33 Antibody (Catalog # AF3626)
  ELISA Detection: Mouse IL-33 Biotinylated Antibody (Catalog # BAF3626)
  Standard:Recombinant Mouse IL-33 (Catalog # 3626-ML)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-33 Antibody [Biotin]

• C9orf26
• C9orf26chromosome 9 open reading frame 26 (NF-HEV)
• DKFZp586H0523
• DVS27
• DVS27-related protein
• IL1F11
• IL-1F11
• IL33
• IL-33
• interleukin 33
• Interleukin-1 family member 11
• interleukin-33
• NFHEV
• NF-HEV
• NF-HEVNFEHEV
• Nuclear factor from high endothelial venules
• RP11-575C20.2

Background

IL-33, also known as NF-HEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription (1-3). DVS 27 was identifed as a gene that is upregulated in vasospastic cerebral arteries (1). NF-HEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues (2). IL-33 was identified based on sequence and structural homology with IL-1 family cytokines (3). DVS 27, NF-HEV, and IL-33 share 100% amino acid (aa) sequence identity. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL-1 beta stimulation (1, 3). Similar to IL-1, IL-33 can be cleaved in vitro by caspase-1, generating an N-terminal fragment that is slightly shorter than the C-terminal fragment (3, 4). The N-terminal portion of full length IL-33 contains a predicted bipartite nuclear localization sequence and a homeodomain-like helix-turn-helix DNA binding domain. By immunofluorescence, full length IL-33 localizes to the nucleus in HUVECs and transfectants (2). The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling through mast cell IL-1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses (3, 5-7). A ternary signaling complex is formed by the subsequent association of IL-33 and ST2L with IL-1R AcP (8). Stimulation of Th2 polarized lymphocytes with mature IL-33 in vitro induces IL-5 and IL-13 secretion (3). In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues (3). Full length and mature mouse IL-33 share approximately 55% and 90% aa sequence identity with human and rat IL-33, respectively. Mouse IL-33 shares less than 25% aa sequence identity with other IL-1 family proteins.

1. Onda, H. et al. (1999) J. Cereb. Blood Flow Metab. 19:1279.
2. Baekkevold, E.S. et al. (2003) Am. J. Pathol. 163:69.
3. Schmitz, J. et al. (2005) Immunity 23:479.
4. Black, R.A. et al. (1989) J. Biol. Chem. 264:5323.
5. Xu, D. et al. (1998) J. Exp. Med. 187:787.
6. Lohning, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6930.
7. Dinarello, C.A. (2005) Immunity 23:461.
8. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-33 Antibody (BAF3626)(12)

Publications using BAF3626

• Ming Zhao, Kaiqun Ren, Xiwen Xiong, Yue Xin, Yujie Zou, Jason C. Maynard et al. Epithelial STAT6 O-GlcNAcylation drives a concerted anti-helminth alarmin response dependent on tuft cell hyperplasia and Gasdermin C Immunity 4/5/2022 [PMID: 35385697]
• Ming Zhao; Kaiqun Ren; Xiwen Xiong; Yue Xin; Yujie Zou; Jason C. Maynard et al. Epithelial STAT6 O-GlcNAcylation drives a concerted anti-helminth alarmin response dependent on tuft cell hyperplasia and Gasdermin C Immunity 2022-04-05 [PMID: 35385697] (Immunohistochemistry, Western Blot, Mouse, Transgenic Mouse)
• BGC Griffith, R Upstill-Go, H Brunton, GR Grimes, AV Biankin, B Serrels, A Byron, MC Frame FAK regulates IL-33 expression by controlling chromatin accessibility at c-Jun motifs Scientific Reports, 2021-01-08;11(1):229. 2021-01-08 [PMID: 33420223] (Western Blot, Human)
• R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3 Cell. Physiol. Biochem., 2017-08-09;42(5):1961-1972. 2017-08-09 [PMID: 28793286] (ELISA Development (Detection), Mouse)
• Denney L, Byrne A, Shea T, Buckley J, Pease J, Herledan G, Walker S, Gregory L, Lloyd C Pulmonary Epithelial Cell-Derived Cytokine TGF-beta1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function. Immunity, 2015-11-17;43(5):945-58. 2015-11-17 [PMID: 26588780] (ELISA Development (Detection), Mouse)
• Kim L, Morita R, Kobayashi Y, Eisenbarth S, Lee C, Elias J, Eynon E, Flavell R AMCase is a crucial regulator of type 2 immune responses to inhaled house dust mites. Proc Natl Acad Sci U S A, 2015-05-18;112(22):E2891-9. 2015-05-18 [PMID: 26038565] (ELISA Development (Detection), Mouse)
• Wu J, Carlock C, Zhou C, Nakae S, Hicks J, Adams H, Lou Y IL-33 is required for disposal of unnecessary cells during ovarian atresia through regulation of autophagy and macrophage migration. J Immunol, 2015-01-23;194(5):2140-7. 2015-01-23 [PMID: 25617473] (Western Blot, Mouse)
• Talabot-Ayer D, Martin P, Vesin C, Seemayer C, Vigne S, Gabay C, Palmer G Severe neutrophil-dominated inflammation and enhanced myelopoiesis in IL-33-overexpressing CMV/IL33 mice. J Immunol, 2014-12-10;194(2):750-60. 2014-12-10 [PMID: 25505285] (Western Blot, Mouse)
• Carlock C, Wu J, Zhou C, Tatum K, Adams H, Tan F, Lou Y Unique temporal and spatial expression patterns of IL-33 in ovaries during ovulation and estrous cycle are associated with ovarian tissue homeostasis. J Immunol, 2014-05-23;193(1):161-9. 2014-05-23 [PMID: 24860190] (Western Blot, Mouse)
• Palm N, Rosenstein R, Yu S, Schenten D, Florsheim E, Medzhitov R Bee venom phospholipase A2 induces a primary type 2 response that is dependent on the receptor ST2 and confers protective immunity. Immunity, 2013-10-24;39(5):976-85. 2013-10-24 [PMID: 24210353] (ELISA Development (Detection), Human, Mouse)
• Alice S Kaanta, Carl Virtanen, Laura M Selfors, Joan S Brugge, Benjamin G Neel Evidence for a multipotent mammary progenitor with pregnancy-specific activity Breast Cancer Research 2013-08-15 [PMID: 23947835] (Flow Cytometry, Mouse)
• Hudson CA, Christophi GP, Gruber RC, Wilmore JR, Lawrence DA, Massa PT Induction of IL-33 expression and activity in central nervous system glia. J. Leukoc. Biol., 2008-06-13;84(3):631-43. 2008-06-13 [PMID: 18552204] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: IL33
• Uniprot:
  • Q8BVZ5()