IL-32 Antibody - BSA Free

Western Blot: IL-32 Antibody - BSA Free [NBP1-76684] - Figure 1 Western Blot Validation with Recombinant ProteinLoading: 30 ng of human IL-32 recombinant protein per lane.Antibodies: IL-32, (Lane 1: 0.5 u/mL, Lane 2: 1 u/mL, Lane 3: 2 u/mL and Lane 4: 4 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Observed at around 17kD.

Immunohistochemistry: IL-32 Antibody - BSA Free [NBP1-76684] - Validation of IL-32 in Human Spleen Tissue.Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-32 antibody at 10 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunocytochemistry/ Immunofluorescence: IL-32 Antibody - BSA Free [NBP1-76684] - Figure 4 Immunofluorescence Validation of IL-32 in Human Spleen CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed human spleen cells labeling IL-32 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Immunohistochemistry: IL-32 Antibody - BSA Free [NBP1-76684] - Figure 5 Immunohistochemistry Validation of IL-32 in Mouse Spleen Tissue Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-IL-32 antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Western Blot: IL-32 Antibody - BSA Free [NBP1-76684] - Validation in Human Spleen Lysate, showing two isoforms of IL-32Loading: 15 ug of lysates per lane.Antibodies: IL-32, (lane 1: 2 ug/mL and lane 2: 4 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

IL-32 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit IL-32 Antibody - BSA Free (NBP1-76684) is a polyclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. Anti-IL-32 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human IL-32. The immunogen is located within the last 50 amino acids of IL-32. Amino Acid Sequence: YGAPRGDKEELTPQK
• Specificity: This antibody detects all isoforms of IL-32. Human IL-32 has 7 isoforms, including isoform 1 (234aa, 27 kDa), isoform 2 (188aa, 22 kDa), isoform 3 (178aa, 21 kDa), isoform 4 (131aa, 15 kDa), isoform 5 (168aa, 19 kDa), isoform 6 (179aa, 21 kDa) and isoform 7 (148aa, 17 kDa). NBP1-76684 can detect all human isoforms.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IL32
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2-10 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Western Blot 0.5 - 4 ug/mL
• Application Notes: Use in FLOW reported in scientific literature (PMID: 30641867).
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  IL-32 Peptide (NBP1-76684PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for IL-32 Antibody - BSA Free

• IL32
• IL-32
• IL-32alpha
• IL-32beta
• interleukin 32
• interleukin-32 theta
• interleukin-32
• natural killer cell transcript 4
• Natural killer cells protein 4
• NK4
• NK4IL-32delta
• TAIF
• TAIFa
• TAIFb
• TAIFc
• TAIFd
• TAIFIL-32gamma
• Tumor necrosis factor alpha-inducing factor

Background

Interleukin-32 (IL-32) was initially identified as a transcript (NK4) that is selectively expressed in lymphocytes and NK cells and whose expression is increased following activation by IL-2. It was later re-isolated from an IL-18-treated lung carcinoma cell line and re-named IL-32. IL-32 is unusual in that it does not share sequence homology with known cytokine families and is highly expressed in immune tissues, existing in at least four differentially spliced isoforms. Because treatment of human monocytic and mouse macrophage cells with IL-32 induces several proinflammatory cytokines such as TNF-alpha, IL-8 and MIP-2, and because it is also induced in human peripheral lymphocyte cells after mitogen stimulation and in epithelial cells by IFNgamma, it has been suggested that IL-32 may play a role in autoimmune and inflammatory diseases such as rheumatoid arthritis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-32 Antibody (NBP1-76684)(3)

Publications using NBP1-76684

• Frew John W, Hawkes Jason E, Krueger James G A systematic review and critical evaluation of immunohistochemical associations in hidradenitis suppurativa. F1000Research 2019-06-25 [PMID: 31281635] (IF/IHC, Human)
• Casu Beatrice, Dondero Alessandra, Regis Stefano et al. Novel Immunoregulatory Functions of IL-18, an Accomplice of TGF-beta 1. Scientific Reports 2019-01-11 [PMID: 30641867] (FLOW, Human)
• Thomi R, Yerly D, Yawalkar N et al. Interleukin-32 is highly expressed in lesions of hidradenitis suppurativa. Br. J. Dermatol. 2017-03-16 [PMID: 28301691] (ELISA, Human)

Review for IL-32 Antibody (NBP1-76684) (1)

Average Rating: 2

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Jan Martinek IHC, IF Human 07/27/2021

Summary

• Application: Other
• Sample Tested: Human Tonsil tissue
• Species: Human
• Lot: 3749-1703

Comments

• Comments: PFA fixed sample ***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***

Bioinformatics

• Gene Symbol: IL32
• Entrez:
  • 9235(Human)
• Uniprot:
  • P24001()