| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3040-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76684-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB30401-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82560-25ul | |||
![Western Blot: IL-32 Antibody - BSA Free [NBP1-76684] - Figure 1 Western Blot Validation with Recombinant ProteinLoading: 30 ng of human IL-32 recombinant protein per lane.Antibodies: IL-32, (Lane 1: 0.5 u/mL, Lane 2: 1 u/mL, Lane 3: 2 u/mL and Lane 4: 4 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Observed at around 17kD.](http://images.novusbio.com/fullsize/nbp1-76684_rabbit-polyclonal-il-32-antibody-271120241958486.gif "Western Blot: IL-32 Antibody - BSA Free [NBP1-76684] - Figure 1 Western Blot Validation with Recombinant ProteinLoading: 30 ng of human IL-32 recombinant protein per lane.Antibodies: IL-32, (Lane 1: 0.5 u/mL, Lane 2: 1 u/mL, Lane 3: 2 u/mL and Lane 4: 4 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Observed at around 17kD.")
![Immunohistochemistry: IL-32 Antibody - BSA Free [NBP1-76684] - Validation of IL-32 in Human Spleen Tissue.Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-32 antibody at 10 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76684_rabbit-polyclonal-il-32-antibody-27112024195846.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-32 Antibody - BSA Free [NBP1-76684] - Figure 4 Immunofluorescence Validation of IL-32 in Human Spleen CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed human spleen cells labeling IL-32 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-76684_rabbit-polyclonal-il-32-antibody-2711202419261817.jpg)
![Immunohistochemistry: IL-32 Antibody - BSA Free [NBP1-76684] - Figure 5 Immunohistochemistry Validation of IL-32 in Mouse Spleen Tissue Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-IL-32 antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76684_rabbit-polyclonal-il-32-antibody-2711202420115519.gif)
![Western Blot: IL-32 Antibody - BSA Free [NBP1-76684] - Validation in Human Spleen Lysate, showing two isoforms of IL-32Loading: 15 ug of lysates per lane.Antibodies: IL-32, (lane 1: 2 ug/mL and lane 2: 4 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76684_rabbit-polyclonal-il-32-antibody-2711202420333812.gif)
![N/A IL-32 [Biotin]](http://images.novusbio.com/fullsize2/DATA_IL32_DY304005_ELISA_2422.jpg "N/A IL-32 [Biotin]")
![Western Blot: IL-32 Antibody [NBP1-82560] - Analysis in control (vector only transfected HEK293T lysate) and IL32 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/IL-32-Antibody-Western-Blot-NBP1-82560-img0009.jpg "Western Blot: IL-32 Antibody [NBP1-82560] - Analysis in control (vector only transfected HEK293T lysate) and IL32 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: IL-32 Antibody [NBP1-82560] - Staining of human tonsil shows moderate to strong cytoplasmic positivity in non - germinal center cells.](http://images.novusbio.com/fullsize/IL-32-Antibody-Immunohistochemistry-Paraffin-NBP1-82560-img0013.jpg)
![Immunohistochemistry-Paraffin: IL-32 Antibody [NBP1-82560] - Staining of human lymph node shows distinct cytoplasmic positivity in subsets of lymphoid cells.](http://images.novusbio.com/fullsize/IL-32-Antibody-Immunohistochemistry-Paraffin-NBP1-82560-img0008.jpg)
![Immunohistochemistry-Paraffin: IL-32 Antibody [NBP1-82560] - Staining of human gastrointestinal shows moderate to strong cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/IL-32-Antibody-Immunohistochemistry-Paraffin-NBP1-82560-img0010.jpg)
![Immunohistochemistry-Paraffin: IL-32 Antibody [NBP1-82560] - Staining of human skin shows strong cytoplasmic positivity in Langerhans cells.](http://images.novusbio.com/fullsize/IL-32-Antibody-Immunohistochemistry-Paraffin-NBP1-82560-img0012.jpg)
![Immunohistochemistry-Paraffin: IL-32 Antibody [NBP1-82560] - Staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.](http://images.novusbio.com/fullsize/IL-32-Antibody-Immunohistochemistry-Paraffin-NBP1-82560-img0011.jpg)
![Western Blot: IL32 Overexpression Lysate (Adult Normal) [NBL1-11952] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IL32.](http://images.novusbio.com/fullsize/IL32-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11952-img0002.jpg "Western Blot: IL32 Overexpression Lysate (Adult Normal) [NBL1-11952] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IL32.")
![ELISA: Human IL-32 ELISA Kit (Colorimetric) [NBP3-06775] - Samples were spiked with high concentrations of Human IL-32 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-06775_human-il-32-elisa-kit-colorimetric-132202416204013.jpg "ELISA: Human IL-32 ELISA Kit (Colorimetric) [NBP3-06775] - Samples were spiked with high concentrations of Human IL-32 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human IL-32 ELISA Kit (Colorimetric) [NBP3-06775] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-06775_human-il-32-elisa-kit-colorimetric-4320249133268.jpg)
![Western Blot: IL32 Overexpression Lysate (Adult Normal) [NBP2-08698] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IL32.](http://images.novusbio.com/fullsize/IL32-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08698-img0001.jpg "Western Blot: IL32 Overexpression Lysate (Adult Normal) [NBP2-08698] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IL32.")
![Western Blot: IL32 Overexpression Lysate (Adult Normal) [NBP2-09142] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IL32.](http://images.novusbio.com/fullsize/IL32-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09142-img0001.jpg "Western Blot: IL32 Overexpression Lysate (Adult Normal) [NBP2-09142] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IL32.")
![Western Blot: IL32 Overexpression Lysate (Adult Normal) [NBP2-08699] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IL32.](http://images.novusbio.com/fullsize/IL32-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08699-img0001.jpg "Western Blot: IL32 Overexpression Lysate (Adult Normal) [NBP2-08699] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IL32.")