IL-32 Antibody

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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IL-32 Antibody Summary

Immunogen
E. coli-derived recombinant human IL‑32 alpha
Cys2-Lys131
Accession # NP_001012651
Specificity
Detects human IL-32 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL32
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF3040 in the following application:

Publications
Read Publications using
AF3040 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-32 Antibody

  • IL32
  • IL-32
  • IL-32alpha
  • IL-32beta
  • interleukin 32
  • interleukin-32 theta
  • interleukin-32
  • natural killer cell transcript 4
  • Natural killer cells protein 4
  • NK4
  • NK4IL-32delta
  • TAIF
  • TAIFa
  • TAIFb
  • TAIFc
  • TAIFd
  • TAIFIL-32gamma
  • Tumor necrosis factor alpha-inducing factor

Background

Interleukin 32 (IL-32) is an N-glycosylated cytokine that is upregulated by inflammatory stimulation in monocytes, NK cells, epithelial cells, and pancreatic myofibroblasts (1-5). It cooperates with these stimuli to promote the expression of other proinflammatory molecules such as TNF-alpha, IL-6, IL-1 beta, IL-1 alpha, and CXCL8/IL‑8 (5-7). The longest of several IL-32 splicing variants is the 20-25 kDa gamma isoform which is also known as natural killer cell transcript 4 (NK4) (8, 9). The alpha isoform (IL-32 alpha ) lacks a portion of the putative signal peptide as well as 57 aa from the C-terminal region. IL-32 alpha is less potent than IL-32 beta, gamma, or δ at inducing the expression of proinflammatory molecules in peripheral blood mononuclear cells (PBMC) (8, 10). Neutrophil-derived Proteinase 3 (PR3) cleaves IL-32 alpha between Thr57 and Val58, a cleavage site that is retained in other IL-32 isoforms (11). The N-terminal fragment of PR3-cleaved IL-32 alpha shows increased potency at inducing CXCL2/MIP-2 and CXCL8 expression in PBMC relative to uncleaved IL-32 alpha (11, 12). IL-32 is highly expressed by colonic epithelial cells in inflammatory bowel disease and Crohn’s disease, rheumatoid arthritis synovium, and ductal epithelial cells in chronic pancreatitis and pancreatic cancer (5, 13-15). IL-32 inhibits HIV-1 replication in vitro, and it is elevated in the serum of HIV-1 patients (16, 17).
  1. Netea, M.G. et al. (2006) PloS Med. 3:e277.
  2. Nold-Petry, C.A. et al. (2009) Proc. Natl. Acad. Sci. USA 106:3883.
  3. Li, W. et al. (2009) Eur. J. Immunol. 39:1019.
  4. Nishida, A. et al. (2008) Am. J. Physiol. Gastrointest. Liver Physiol. 294:G831.
  5. Shoda, H. et al. (2006) Arthritis Res. Ther. 8:R166.
  6. Netea, M.G. et al. (2005) Proc. Natl. Acad. Sci. USA 102:16309.
  7. Hong, J. et al. (2010) Cytokine 49:171.
  8. Kim, S-H. et al. (2005) Immunity 22:131.
  9. Dahl, C.A. et al. (1992) J. Immunol. 148:597.
  10. Choi, J-D. et al. (2009) Immunology 126:535.
  11. Novick, D. et al. (2006) Proc. Natl. Acad. Sci. USA 103:3316.
  12. Kim, S. et al. (2008) BMB Rep. 41:814.
  13. Shioya, M. et al. (2007) Clin. Exp. Immunol. 149:480.
  14. Joosten, L.A.B. et al. (2006) Proc. Natl. Acad. Sci. USA 103:3298.
  15. Nishida, A. et al. (2009) J. Biol. Chem. 284:17868.
  16. Rasool, S.T. et al. (2008) Immunol. Lett. 117:161.
  17. Nold, M.F. et al. (2008) J. Immunol. 181:557.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-32 Antibody (AF3040)(11)

We have publications tested in 2 confirmed species: Human, Transgenic Mouse.

We have publications tested in 6 applications: ELISA Development, Electrochemiluminescent Assay, IHC, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
ELISA Development
(1)
Electrochemiluminescent Assay
(1)
IHC
(2)
IHC-P
(1)
Immunoprecipitation
(1)
Western Blot
(4)
All Applications
Filter By Species
Human
(10)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF3040 Applications Species
JC Dos Santos, AM Barroso de, MV Teodoro Si, B Cirovic, LCJ de Bree, MSMA Damen, SJCFM Moorlag, RS Gomes, MM Helsen, M Oosting, ST Keating, A Schlitzer, MG Netea, F Ribeiro-Di, LAB Joosten β-Glucan-Induced Trained Immunity Protects against Leishmania braziliensis Infection: a Crucial Role for IL-32 Cell Rep, 2019;28(10):2659-2672.e6. 2019 [PMID: 31484076] (IHC-P, Transgenic Mouse) IHC-P Transgenic Mouse
MSMA Damen, K Schraa, L Tweehuysen, AA den Broede, MG Netea, CD Popa, LAB Joosten Genetic variant in IL-32 is associated with the ex vivo cytokine production of anti-TNF treated PBMCs from rheumatoid arthritis patients Sci Rep, 2018;8(1):14050. 2018 [PMID: 30232372] (Human) Human
RJ Palstra, E de Crignis, MD Röling, T van Staver, TW Kan, W van Ijcken, YM Mueller, PD Katsikis, T Mahmoudi Allele-specific long-distance regulation dictates IL-32 isoform switching and mediates susceptibility to HIV-1 Sci Adv, 2018;4(2):e1701729. 2018 [PMID: 29507875] (Western Blot, Human) Western Blot Human
Kang J, Park Y, Kim M, Lee D, Bak Y, Ham S, Park S, Hong J, Yoon D Interleukin (IL)-32beta-mediated CCAAT/enhancer-binding protein alpha (C/EBPalpha) phosphorylation by protein kinase Cdelta (PKCdelta) abrogates the inhibitory effect of C/EBPalpha on IL-10 production. J Biol Chem, 2013;288(33):23650-8. 2013 [PMID: 23814099] (Immunoprecipitation, Human) Immunoprecipitation Human
Sakitani K, Hirata Y, Hayakawa Y, Serizawa T, Nakata W, Takahashi R, Kinoshita H, Sakamoto K, Nakagawa H, Akanuma M, Yoshida H, Maeda S, Koike K Role of interleukin-32 in Helicobacter pylori-induced gastric inflammation. Infect Immun, 2012;80(11):3795-803. 2012 [PMID: 22890997] (Western Blot, Human) Western Blot Human
Marcondes AM, Li X, Tabellini L, Bartenstein M, Kabacka J, Sale GE, Hansen JA, Dinarello CA, Deeg HJ Inhibition of IL-32 activation by alpha-1 antitrypsin suppresses alloreactivity and increases survival in an allogeneic murine marrow transplantation model. Blood, 2011;118(18):5031-9. 2011 [PMID: 21900190] (Western Blot, Human) Western Blot Human
Heinhuis B, Koenders MI, van de Loo FA, Netea MG, van den Berg WB, Joosten LA Inflammation-dependent secretion and splicing of IL-32{gamma} in rheumatoid arthritis. Proc. Natl. Acad. Sci. U.S.A., 2011;108(12):4962-7. 2011 [PMID: 21383200] (ELISA Development, Human) ELISA Development Human
Zepp JA, Nold-Petry CA, Dinarello CA, Nold MF Protection from RNA and DNA viruses by IL-32. J. Immunol., 2011;186(7):4110-8. 2011 [PMID: 21346229] (Electrochemiluminescent Assay, Human) Electrochemiluminescent Assay Human
Hasegawa H, Thomas HJ, Schooley K, Born TL Native IL-32 is released from intestinal epithelial cells via a non-classical secretory pathway as a membrane-associated protein. Cytokine, 2011;53(1):74-83. 2011 [PMID: 20926308] (Western Blot, Human) Western Blot Human
Nishida A, Andoh A, Inatomi O, Fujiyama Y Interleukin-32 expression in the pancreas. J. Biol. Chem., 2009;284(26):17868-76. 2009 [PMID: 19386602] (IHC, Human) IHC Human
Show All 11 Publications.

Review for IL-32 Antibody (AF3040) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF3040:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Anonymous
WB Human 01/07/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22890997
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22890997

Details

Detection NotesSee PMID 22890997

Comments

CommentsPublished: See PMID 22890997

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Secondary Antibodies

 

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Other Available Formats

Biotin BAF3040

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Recent Reviews

5
5
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Anonymous
01/07/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol IL32
Uniprot