IL-32 alpha Antibody (373802)

Images

 
Western blot shows lysates of human peripheral blood mononuclear cells (PBMC) treated (+) with 200 ng/mL Ionomycin and 10 ng/mL PMA for 72 hours. PVDF Membrane was probed with 2 µg/mL of Human IL‑32 alpha Monoclonal ...read more
IL‑32 alpha was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human IL‑32 alpha Monoclonal Antibody (Catalog # MAB30401) at 10 µg/mL for 3 hours at room temperature. Cells ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
373802
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

IL-32 alpha Antibody (373802) Summary

Immunogen
E. coli-derived recombinant human IL‑32 alpha
Phe3-Lys131
Accession # NP_001012651
Specificity
Detects the alpha isoform of IL-32 in direct ELISAs. In direct ELISAs, less than 15% cross-reactivity with recombinant human (rh) IL-32 beta and rhIL‑32 gamma is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
IL32
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Immunocytochemistry 8-25 ug/mL
Publications
Read Publications using
MAB30401 in the following applications:

  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-32 alpha Antibody (373802)

  • IL32 alpha
  • IL-32 alpha
  • IL32
  • IL-32alpha
  • IL-32beta
  • IL-32delta
  • IL-32gamma
  • interleukin 32
  • NK4
  • TAIF
  • TAIFa
  • TAIFb
  • TAIFc
  • TAIFd

Background

IL-32 alpha is the shortest and most abundant of four potential splice variants of the proinflammatory cytokine IL-32 (previously called NK4) with a predicted unmodified size of 15 kDa. Potential modifications include myristoylation and N-glycosylation. Transfected IL-32 alpha was more likely to be cell-associated as compared to IL-32 beta, suggesting an intracellular function. IL-32 is induced by mitogens in peripheral lymphocytes, by IFN‑ gamma in epithelial cells, or by IL-12 with IL-18 in NK cells and in turn Induces cytokine expression. No ortholog has been found in mouse.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-32 alpha Antibody (MAB30401)(3)

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Bioinformatics

Gene Symbol IL32
Uniprot