Mouse IL-23A/IL-23 P19 ELISA Kit (Colorimetric) Summary
| Description |
Quality control test: Standard curve. Il23a/Il12b (Mouse) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of mouse Il23a/mouse Il12b in serum, plasma, body fluids, tissue lysates or cell culture supernates. |
| Standard Curve Range |
31.2 to 2000 pg/mL |
| Sensitivity |
<2 pg/mL |
| Assay Type |
Sandwich ELISA |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
IL23A |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Useful for quantification. |
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Kit Components
|
Components
|
- ABC diluent buffer: 12ml.
- Antibody diluent buffer: 12ml.
- Avidin-Biotin-Peroxidase Complex (ABC): 130µl, dilution 1:100.
- Biotinylated anti- mouse IL-23 antibody: 130µl, dilution 1:100.
- Lyophilized recombinant mouse IL-23 standard: 10ng/tube×2.
- One 96-well plate precoated with anti- mouse IL-23 antibody.
- Sample diluent buffer: 30 ml
- TMB color developing agent: 10ml.
- TMB stop solution: 10ml.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Mouse IL-23A/IL-23 P19 ELISA Kit (Colorimetric)
Background
The IL23 P19 gene encodes a subunit of the heterodimeric cytokine interleukin 23 (IL23). IL23 is composed of this protein and the p40 subunit of interleukin 12 (IL12B). The receptor of IL23 is formed by the beta 1 subunit of IL12 (IL12RB1) and an IL23 specific su
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for IL-23A/IL-23 P19 ELISA Kit (KA1409). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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