Recombinant Mouse IL-22 Protein

SDS-Page: Mouse IL-22 Protein [NBP2-35122]

• Reactivity: Mu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Mouse IL-22 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 146 amino acids corresponding to IL-22 Source: E. coli

  Uniprot ID: Q9JJY9

  Amino Acid Sequence: LPVNTRCKLE VSNFQQPYIV NRTFMLAKEA SLADNNTDVR LIGEKLFRGV SAKDQCYLMK QVLNFTLEDV LLPQSDRFQP YMQEVVPFLT KLSNQLSSCH ISGDDQNIQK NVRRLKETVK KLGESGEIKA IGELDLLFMS LRNACV

• Details of Functionality: IL-22 protein is fully biologically active when compared to standard. The ED50 as determined by inducing IL-10 secretion of human COLO 205 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 x 10^6 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IL22
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of IL-22 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 16.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 1 x PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Mouse IL-22 Protein

• Cytokine Zcyto18
• IL-10-related T-cell-derived inducible factor
• IL22
• IL-22
• IL-D110
• IL-TIF
• ILTIFIL-10-related T-cell-derived-inducible factor
• IL-TIFMGC79382
• interleukin 22
• interleukin-22
• MGC79384
• TIFa
• TIFIL-23
• zcyto18

Background

IL-22 is belonging to IL-10 family of regulatory cytokines which includes IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. Members of this family share partial homology in their amino acid sequences, but they are dissimilar in their biological functions. Produced by T lymphocytes and dendritic cells, IL-10 contributes to the inflammatory response in vivo. IL-22 signals through CRF2-4 and IL-22. It along with IL-17 is rapidly produced by splenic LTi-like cells and can be also produced by Th17 cells and likely plays a role in the coordinated response of both adaptive and innate immune systems. Recombinant mouse interleukin-22 contains 147 amino acids and it has approximately 81 % and 92 % amino acid sequence identity with human and rat IL-22.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Review for IL-22 Recombinant Protein (NBP2-35122) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Sweta Ghosh Media additive for protein or antibody production 04/03/2024

Summary

• Application: Media additive for protein or antibody production
• Lot: K12122011

Bioinformatics

• Gene Symbol: IL22
• Uniprot:
  • Q9JJY9()
  • Q9JJY9()
  • Q9JJY9()