IL-22 Antibody (924324) [Unconjugated]

Recombinant Mouse IL‑22 stimulates IL-10 secretion in the COLO 205 human colorectal adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by the Human IL-10 DuoSet ELISA Development Kit (Catalog # DY217B). IL-10 Secretion elicited by Recombinant Mouse IL‑22 (500 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑22 Monoclonal Antibody (Catalog # MAB5821). The ND50 is typically 0.5-6 µg/mL.

• Reactivity: Mu
• Applications: B/N
• Clone: 924324
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

IL-22 Antibody (924324) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse IL-22
  Leu34-Val179
  Accession # Q9JJY9
• Specificity: Detects mouse IL-22 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: IL22
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.5-6 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-22 Antibody (924324) [Unconjugated]

• Cytokine Zcyto18
• IL-10-related T-cell-derived inducible factor
• IL-21
• IL22
• IL-22
• IL-22IL21
• IL-D110
• IL-TIF
• ILTIFIL-10-related T-cell-derived-inducible factor
• IL-TIFMGC79382
• interleukin 21
• interleukin 22
• interleukin-22
• MGC79384
• TIFa
• TIFIL-23
• zcyto18

Background

Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Mouse IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with human IL-22 and IL-10, respectively. The mouse IL-22 gene is localized to chromosome 10. Although it exists as a single copy gene in many mouse strains, the IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT-1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal mouse T cells upon Con A activation. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.

1. Dumoutier, L. et al. (2000) J. Immunol. 164:1814.
2. Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335.
3. Dumoutier, L. et al. (2000) PNAS 97:10144.
4. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-22 Antibody (MAB5821)(1)

Publication using MAB5821

• JM Garth, KM Reeder, MS Godwin, JJ Mackel, CW Dunaway, JP Blackburn, C Steele IL-33 Signaling Regulates Innate IL-17A and IL-22 Production via Suppression of Prostaglandin E2 during Lung Fungal Infection J. Immunol., 2017;199(6):2140-2148. 2017 [PMID: 28784844] (Mouse)

Bioinformatics

• Gene Symbol: IL22
• Uniprot:
  • Q9JJY9()