IL-22 Antibody (140301) [Unconjugated]

Mouse splenocytes differentiated to Th17 cells with plate-bound Rat anti-Mouse CD3e monoclonal antibody (MAB484, 5 µg/mL) plus Goat anti-Mouse CD28 (AF483, 5 µg/mL), Recombinant Human TGF-beta 1 (240-B, 10 ng/mL) Recombinant Mouse IL-23 (1887-ML, 20 ng/mL), Recombinant Mouse IL-6 (406-ML, 40 ng/mL), Recombinant Mouse IL-1 beta (401-ML, 10 ng/mL), and Rat anti-Mouse IFN-gamma (MAB485, 10 µg/mL) for 5 days then re-stimulated with PMA (50 ng/ml), Ca2+ Ionomycin (200 ng/ml) and Brefeldin A ( 5 μg/ml) for 4 hours, were stained with APC-conjugated Rat anti-Mouse CD4 Monoclonal Antibody (FAB554A) and (A) Rat Anti-Mouse IL-22 Monoclonal Antibody (Catalog # MAB582) or (B) isotype control antibody (MAB006) followed by PE-conjugated Goat anti-Rat secondary antibody (F0105B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit. (FC012). View our protocol for Staining Intracellular Molecules.

• Reactivity: Mu
• Applications: WB, CyTOF-ready, Flow
• Clone: 140301
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

IL-22 Antibody (140301) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse IL-22
  Leu34-Val179
  Accession # Q9JJY9
• Specificity: Detects mouse IL-22 in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant rat IL-22 is observed, 15% cross-reactivity with recombinant human IL-22 is observed, and no cross-reactivity with recombinant mouse IL-10 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: IL22
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-22 Antibody (140301) [Unconjugated]

• Cytokine Zcyto18
• IL-10-related T-cell-derived inducible factor
• IL22
• IL-22
• IL-D110
• IL-TIF
• ILTIFIL-10-related T-cell-derived-inducible factor
• IL-TIFMGC79382
• interleukin 22
• interleukin-22
• MGC79384
• TIFa
• TIFIL-23
• zcyto18

Background

Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Mouse IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with human IL-22 and IL-10, respectively. The mouse IL-22 gene is localized to chromosome 10. Although it exists as a single copy gene in many mouse strains, the IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta , share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT-1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal mouse T cells upon Con A activation. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.

1. Dumoutier, L. et al. (2000) J. Immunol. 164:1814.
2. Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335.
3. Dumoutier, L. et al. (2000) PNAS 97:10144.
4. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-22 Antibody (MAB582)(3)

Publications using MAB582

• X Jiang, CO Park, J Geddes Swe, MJ Yoo, O Gaide, TS Kupper Dermal ?� T Cells Do Not Freely Re-Circulate Out of Skin and Produce IL-17 to Promote Neutrophil Infiltration during Primary Contact Hypersensitivity PLoS ONE, 2017-01-12;12(1):e0169397. 2017-01-12 [PMID: 28081153] (Flow Cytometry, Mouse)
• De Luca A, Zelante T, D'Angelo C IL-22 defines a novel immune pathway of antifungal resistance. Mucosal Immunol, 2010-05-05;3(4):361-73. 2010-05-05 [PMID: 20445503] (Flow Cytometry, Mouse)
• Radaeva S, Sun R, Pan HN, Hong F, Gao B Interleukin 22 (IL-22) plays a protective role in T cell-mediated murine hepatitis: IL-22 is a survival factor for hepatocytes via STAT3 activation. Hepatology, 2004-05-01;39(5):1332-42. 2004-05-01 [PMID: 15122762] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: IL22
• Uniprot:
  • Q9JJY9()