IL-21 Antibody

Recombinant Canine IL‑21 (Catalog # 5849-ML) induces proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL‑21 (10 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Canine IL‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5849). The ND50 is typically 2-10 µg/mL.

• Reactivity: Ca
• Applications: B/N
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-21 Antibody Summary

• Immunogen: E. coli-derived recombinant canine IL-21
  His18-Ser146
  Accession # NP_001003347
• Specificity: Detects canine IL-21 in direct ELISAs. In direct ELISAs, approximately 35% cross-reactivity with recombinant human IL-21 is observed, and less than 3% cross-reactivity with recombinant mouse IL-21 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: IL21
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 2-10 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-21 Antibody

• CVID11
• IL21
• IL-21
• IL-21Za11interleukin-21
• interleukin 21
• interleukin-21 isoform
• Za11

Background

Interleukin-21 (IL-21) is a 14 kDa (predicted) four-helix-bundle cytokine and member of the IL-15/IL-21 family. It is made by activated CD4 + T cells, activated NKT cells, T helper (Th) cells, and Th17 cells (1-5). Canine IL-21 is synthesized as a 146 amino acid (aa) precursor that contains a 17 aa signal sequence and a 129 aa mature chain. Mature canine IL-21 is 86%, 73%, 66%, and 64% aa identical to mature bovine, human, mouse and rat IL-21, respectively. IL-21 binds to a heterodimeric receptor, which is formed by the common gamma -chain subunit (CD134), shared with IL-2, IL-4, IL-7, IL-9, IL-13, and IL-15 receptors, and its own receptor, IL-21 R, a member of the class I cytokine receptor family (1-2). IL-21 R is expressed on a variety of immune cells, including B cells, T cells, dendritic cells, and NK cells, as well as non-immune cells, such as fibroblasts, epithelial cells, and endothelial cells (1-2, 5). Binding of IL-21 to its receptor leads to the activation of members of the JAK-family protein tyrosine kinases, JAK1 and JAK3, and signal transducer and activator of transcription (Stat) molecules (1). IL-21 regulates activation, proliferation, and survival of both CD4 + T cells and B cells, the functional activity of CD8 + T cells and NK cells, and limits the differentiation of inducible regulatory T cells and counteracts their suppressive properties on effector T cells (1, 5-6). IL-21 also negatively regulates the maturation and function of dendritic cells (1, 5-6). IL-21 also plays a role in many inflammatory and autoimmune diseases, such as Crohn’s disease, Helicobacter pylori-related gastritis, celiac disease, type I diabetes mellitus, psoriasis, rheumatoid arthritis, and systemic lupus erythematosus (1). IL-21 is also involved in controlling chronic viral infections (7).

1. Monteleone, G. et al. (2009) Cytokine Growth Factor Rev. 20:185.
2. Parrish-Novak, J. et al. (2000) Nature 408:57.
3. Coquet, J.M. et al. (2007) J. Immunol.178:2827.
4. Vogelzang, A. et al. (2008) Immunity 29:127.
5. Monteleone, G. et al. (2008) Trends Immunol. 29:290.
6. Spolski, R. et al. (2008) Curr. Opin. Immunol. 20:295.
7. Yi, J.S. et al. (2009) Science 324:1572.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL21
• Uniprot:
  • NP_001003347()