IL-2 R beta Antibody (27302) [Phycoerythrin]

• Applications: Flow
• Clone: 27302
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

IL-2 R beta Antibody (27302) [Phycoerythrin] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑2 R beta
  Ala27-Asp239
  Accession # NP_000869
• Specificity: Detects human IL-2 R beta in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑2 R alpha , rh gamma _c, rhIL‑4 R, or rhIL‑6 R is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL2RB
• Purity Statement: Protein A or G purified from ascites

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Functional IL-2 receptors can exist in two affinity states on cell surfaces, the high affinity complex consisting of heterotrimers of the alpha , beta , and gamma chains and the intermediate affinity complex comprising heterodimers of the beta and gamma chains. Individual beta chains and alpha chains exhibit low affinity IL-2 binding, while the gamma chain alone does not bind IL-2. In addition to their involvement in IL-2 mediated signal transduction, both the beta chain and gamma chain have been shown to be required for IL-15 mediated signaling. IL-2 R beta is a member of the cytokine receptor superfamily. Human IL-2 R beta cDNA encodes a 551 amino acid (aa) precursor Type I membrane protein with a 26 aa signal peptide, a 214 aa extracellular region, a 25 aa transmembrane region and a 286 aa cytoplasmic domain. A soluble IL-2 R beta has been identified in the culture supernatants of a human lymphoid cell line, YT, that displays IL-2 R beta . Soluble IL-2 R beta binds IL-2 with low affinity and is not an effective IL-2 antagonist on cells displaying the high or intermediate affinity IL-2 signaling receptors. Nevertheless, soluble IL-2 R beta binds IL-15 with sufficient affinity to neutralize IL-15 biological activities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL2RB