IL-18R alpha/IL-1 R5 Antibody (70614)

• Reactivity: Hu
• Applications: WB
• Clone: 70614
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IL-18R alpha/IL-1 R5 Antibody (70614) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL‑18 R alpha /IL‑1 R5
  Glu20-Arg329
  Accession # Q13478
• Specificity: Detects human IL‑18 R alpha /IL‑1 R5 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑1 R1, rhIL-1 R2, rhIL-1 R3, rhIL-1 R4, rhIL-1 R6, rhIL-1 R7, rhIL-1 R8, rhIL-1 R9, or rhSIGIRR is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL18R1
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-18R alpha/IL-1 R5 Antibody (70614)

• CD218a
• CD218a;CDw218a;IL18R1;IL18RA;IL-18Ralpha;IL-18R-alpha;IL18Ralpha2;IL1RRP;IL-1Rrp;Interleukin-18 receptor 1
• IL-1 R5
• IL-1 Rrp
• IL-18 R alpha
• IL-18 R1
• IL18R alpha
• IL18R1
• IL-18Ra

Background

Interleukin 18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immunoregulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL-18 R alpha (also termed IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also termed IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R by itself binds IL-18 with low affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL‑18 R alpha and IL‑18 R beta is required for high affinity binding and IL-18 responsiveness. Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate and placenta.

1. Parnet, P. et al. (1996) J. Biol. Chem. 271:3967.
2. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
3. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-18R alpha/IL-1 R5 Antibody (MAB8401)(1)

Publication using MAB8401

• Nold-Petry CA, Nold MF, Nielsen JW, Bustamante A, Zepp JA, Storm KA, Hong JW, Kim SH, Dinarello CA Increased cytokine production in IL-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling (SOCS). J. Biol. Chem., 2009-07-10;0(0):. 2009-07-10 [PMID: 19592492] (Western Blot, Human)

Bioinformatics

• Gene Symbol: IL18R1
• Uniprot:
  • Q13478()