9124-IL) induced IFN‑ gamma secretion in KG-1 human myeloid leukemia cells in the presence of Recombinant Human TNF-alpha (210-TA) as measured by the Human IFN-gamma Quantikine ELISA Kit (DIF50C). The Neutralization Dose (ND₅₀) is typically < 0.500 µg/mL." class="big_lightbox"> 9124-IL) induced IFN‑ gamma secretion in KG-1 human myeloid leukemia cells in the presence of Recombinant Human TNF-alpha (210-TA) as measured by the Human IFN-gamma Quantikine ELISA Kit (DIF50C). The Neutralization Dose (ND₅₀) is typically < 0.500 µg/mL." alt="Human IL‑18 R alpha /IL‑1 R5 Antibody (Catalog # MAB840) neutralizes IL‑18/IL‑1F4 (9124-IL) induced IFN‑ gamma secretion in KG-1 human myeloid leukemia cells in the presence of Recombinant Human TNF-alpha (210-TA) as measured by the Human IFN-gamma Quantikine ELISA Kit (DIF50C). The Neutralization Dose (ND₅₀) is typically < 0.500 µg/mL." class="big_thumb" />

MAB002) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (F0102B) and Mouse Anti-Human CD3 PE-conjugated Monoclonal Antibody (FAB100P). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_lightbox"> MAB002) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (F0102B) and Mouse Anti-Human CD3 PE-conjugated Monoclonal Antibody (FAB100P). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_thumb" />

NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell surface of keratinocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell surface of keratinocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

CTS009) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of keratinocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS009) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of keratinocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="IL‑18 Ra/IL‑1 R5 was detected in immersion fixed frozen sections of human skin using Goat Anti-Human IL‑18 Ra/IL‑1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF840) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of keratinocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

DIF50C). IFN‑ gamma secretion elicited by IL‑18/IL‑1F4 (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑18 Ra/IL‑1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF840). The ND₅₀ is typically 0.1-0.4 µg/mL." class="big_lightbox"> DIF50C). IFN‑ gamma secretion elicited by IL‑18/IL‑1F4 (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑18 Ra/IL‑1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF840). The ND₅₀ is typically 0.1-0.4 µg/mL." class="big_thumb" />

419-ML), Recombinant Mouse IL-18/IL-1F4 stimulates IFN-gamma secretion in activated mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IFN-gamma Quantikine ELISA Kit (Catalog # MIF00). Under these conditions, IFN-gamma secretion elicited by Recombinant Mouse IL-18/IL-1F4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-18 Ra/IL-1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF856). The ND₅₀ is typically 0.2-0.8 µg/mL." class="big_lightbox"> 419-ML), Recombinant Mouse IL-18/IL-1F4 stimulates IFN-gamma secretion in activated mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IFN-gamma Quantikine ELISA Kit (Catalog # MIF00). Under these conditions, IFN-gamma secretion elicited by Recombinant Mouse IL-18/IL-1F4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-18 Ra/IL-1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF856). The ND₅₀ is typically 0.2-0.8 µg/mL." alt="In the presence of Recombinant Mouse IL-12 (0.1 ng/mL, Catalog # 419-ML), Recombinant Mouse IL-18/IL-1F4 stimulates IFN-gamma secretion in activated mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IFN-gamma Quantikine ELISA Kit (Catalog # MIF00). Under these conditions, IFN-gamma secretion elicited by Recombinant Mouse IL-18/IL-1F4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-18 Ra/IL-1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF856). The ND₅₀ is typically 0.2-0.8 µg/mL." class="big_thumb" />

FAB4841P) and either (A) Goat Anti-Mouse IL‑18 R alpha /IL‑1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF856) or (B) Normal Goat IgG Control (Catalog # AB-108-C) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> FAB4841P) and either (A) Goat Anti-Mouse IL‑18 R alpha /IL‑1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF856) or (B) Normal Goat IgG Control (Catalog # AB-108-C) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

419-ML), Recombinant Mouse IL-18/IL-1F4 stimulates IFN-gamma secretion in activated mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IFN-gamma Quantikine ELISA Kit (Catalog # MIF00). Under these conditions, IFN-gamma secretion elicited by Recombinant Mouse IL-18/IL-1F4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-18 Ra/IL-1 R5 Monoclonal Antibody (Catalog # MAB12161). The ND₅₀ is typically 0.2-0.8 µg/mL." class="big_lightbox"> 419-ML), Recombinant Mouse IL-18/IL-1F4 stimulates IFN-gamma secretion in activated mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IFN-gamma Quantikine ELISA Kit (Catalog # MIF00). Under these conditions, IFN-gamma secretion elicited by Recombinant Mouse IL-18/IL-1F4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-18 Ra/IL-1 R5 Monoclonal Antibody (Catalog # MAB12161). The ND₅₀ is typically 0.2-0.8 µg/mL." alt="In the presence of Recombinant Mouse IL-12 (0.1 ng/mL, Catalog # 419-ML), Recombinant Mouse IL-18/IL-1F4 stimulates IFN-gamma secretion in activated mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IFN-gamma Quantikine ELISA Kit (Catalog # MIF00). Under these conditions, IFN-gamma secretion elicited by Recombinant Mouse IL-18/IL-1F4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-18 Ra/IL-1 R5 Monoclonal Antibody (Catalog # MAB12161). The ND₅₀ is typically 0.2-0.8 µg/mL." class="big_thumb" />