IL-18 R beta/IL-1 R7/ACPL Antibody [Biotin]

• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

IL-18 R beta/IL-1 R7/ACPL Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IL‑18 R beta /IL‑1 R7
  Phe20-Glu356
  Accession # Q9Z2B1
• Specificity: Detects mouse IL-18 R beta in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human (rh) IL-18 R beta is observed, 10% cross-reactivity with rhIL-1 R9 is observed, and less than 2% cross-reactivity with recombinant mouse (rm) IL-1 R8, recombinant rat IL-1 R6, rhIL-1 R3, rmIL-1 R1, rmIL-1 RII, and rmIL-18 R is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL18RAP
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

IL-18, originally described as an interferon-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines that has multiple immunoregulatory functions. It has potent IFN-gamma inducing activities and plays a key role in the activation of T helper type 1 (Th1) responses. The functional IL-18 receptor complex consists of two components, the IL-18R alpha (IL-1 R5) and IL-18R beta (also termed IL-1 R7 and AcPL) subunits. Both subunits are members of the IL-1 receptor superfamily. Although IL-18R alpha by itself binds IL-18 with low-affinity and IL-18R beta does not bind IL-18 in vitro, co-expression of IL-18R alpha and IL-18R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18R beta cDNA encodes a 599 amino acid (aa) residue precursor type I membrane protein with a aa signal peptide, a aa extracellular region containing three immunoglobulin-like domains, a single transmembrane domain and a 222 aa cytoplasmic domain. Human and mouse IL-18 R beta share 65% aa sequence identity. The expression of IL-18R beta parallels that of IL-18R alpha and is detected in numerous tissues including lung, spleen, leukocytes and colon.

Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
Okamura, H. et al. (2000) in Cytokine Reference, Vol. 2:1605, Academic Press.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL18RAP