IL-18 R beta/IL-1 R7/ACPL Antibody [Biotin]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

IL-18 R beta/IL-1 R7/ACPL Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IL‑18 R beta /IL‑1 R7
  Phe20-Glu356
  Accession # Q9Z2B1
• Specificity: Detects mouse IL-18 R beta in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human (rh) IL-18 R beta is observed, 10% cross-reactivity with rhIL-1 R9 is observed, and less than 2% cross-reactivity with recombinant mouse (rm) IL-1 R8, recombinant rat IL-1 R6, rhIL-1 R3, rmIL-1 R1, rmIL-1 RII, and rmIL-18 R is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL18RAP
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-18 R beta/IL-1 R7/ACPL Antibody [Biotin]

• Accessory protein-like
• AcPL
• CD218 antigen-like family member B
• CD218b antigen
• CD218b
• CDw218b
• IL-1 R7
• IL-18 R beta
• IL-18 receptor accessory protein
• IL-18 receptor beta
• IL18R beta
• IL-18RAcP
• IL18RAP
• IL18RB
• IL-18Rb
• IL-18Rbeta
• IL-18R-beta
• IL-1R accessory protein-like
• IL1R7
• IL-1R7
• IL-1R-7
• IL-1RAcPL
• interleukin 18 receptor accessory protein
• Interleukin-1 receptor 7
• interleukin-18 receptor accessory protein
• Interleukin-18 receptor accessory protein-like
• Interleukin-18 receptor beta
• MGC120589
• MGC120590

Background

IL-18, originally described as an interferon-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines that has multiple immunoregulatory functions. It has potent IFN-gamma inducing activities and plays a key role in the activation of T helper type 1 (Th1) responses. The functional IL-18 receptor complex consists of two components, the IL-18R alpha (IL-1 R5) and IL-18R beta (also termed IL-1 R7 and AcPL) subunits. Both subunits are members of the IL-1 receptor superfamily. Although IL-18R alpha by itself binds IL-18 with low-affinity and IL-18R beta does not bind IL-18 in vitro, co-expression of IL-18R alpha and IL-18R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18R beta cDNA encodes a 599 amino acid (aa) residue precursor type I membrane protein with a aa signal peptide, a aa extracellular region containing three immunoglobulin-like domains, a single transmembrane domain and a 222 aa cytoplasmic domain. Human and mouse IL-18 R beta share 65% aa sequence identity. The expression of IL-18R beta parallels that of IL-18R alpha and is detected in numerous tissues including lung, spleen, leukocytes and colon.

Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
Okamura, H. et al. (2000) in Cytokine Reference, Vol. 2:1605, Academic Press.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-18 R beta/IL-1 R7/ACPL Antibody (BAF1520)(3)

Publications using BAF1520

• W Zhuang, C Wang, X Shi, S Qiu, S Zhang, B Xu, M Chen, W Jiang, H Dong, Y Qiao MCMV triggers ROS/NLRP3-associated inflammasome activation in the inner ear of mice and cultured spiral ganglion neurons, contributing to sensorineural hearing loss Int. J. Mol. Med., 2018-03-06;0(0):. 2018-03-06 [PMID: 29512778] (Western Blot, Mouse)
• Silvia Alboni, Claudia Montanari, Cristina Benatti, Johanna M.C. Blom, Maria Luisa Simone, Nicoletta Brunello et al. Constitutive and LPS-regulated expression of interleukin-18 receptor beta variants in the mouse brain Brain, Behavior, and Immunity 2011-03-01 [PMID: 21111041]
• Li W, Kashiwamura S, Ueda H, Sekiyama A, Okamura H Protection of CD8+ T cells from activation-induced cell death by IL-18. J. Leukoc. Biol., 2007-03-30;82(1):142-51. 2007-03-30 [PMID: 17400610] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: IL18RAP
• Uniprot:
  • Q9Z2B1()