LNK/SH2B3 Antibody (OTI2D8)

Western Blot: LNK Antibody (2D8) [NBP2-00641] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LNK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LNK.

Immunocytochemistry/Immunofluorescence: LNK Antibody (2D8) [NBP2-00641] Staining of COS7 cells transiently transfected by pCMV6-ENTRY LNK.

Immunohistochemistry-Paraffin: LNK Antibody (2D8) [NBP2-00641] - Staining of paraffin-embedded Human pancreas tissue using anti-LNK mouse monoclonal antibody.

Flow Cytometry: LNK Antibody (2D8) [NBP2-00641] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LNK antibody, and then analyzed by flow cytometry.

Immunohistochemistry-Paraffin: LNK Antibody (2D8) [NBP2-00641] - Staining of paraffin-embedded Human Kidney tissue using anti-LNK mouse monoclonal antibody.

Flow Cytometry: LNK Antibody (2D8) [NBP2-00641] - Analysis of Hela cells, using anti-LNK antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI2D8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.37 mg/ml

LNK/SH2B3 Antibody (OTI2D8) Summary

• Immunogen: Full length human recombinant protein of human SH2B3 (NP_005466) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SH2B3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 63 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.37 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LNK/SH2B3 Antibody (OTI2D8)

• IDDM20
• LNK
• LNKSH2B adapter protein 3
• Lymphocyte adapter protein
• Lymphocyte-specific adapter protein Lnk
• SH2B adaptor protein 3
• SH2B3
• Signal transduction protein Lnk

Background

This gene encodes a member of the SH2B adaptor family of proteins, which are involved in a range of signaling activities by growth factor and cytokine receptors. The encoded protein is a key negative regulator of cytokine signaling and plays a critical role in hematopoiesis. Mutations in this gene have been associated with susceptibility to celiac disease type 13 and susceptibility to insulin-dependent diabetes mellitus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SH2B3
• Entrez:
  • 10019(Human)