IL-17RA/IL-17R Antibody (657603)

EL‑4 mouse lymphoblast cell line was stained with Rat Anti-Mouse IL‑17 RA/IL‑17 R Monoclonal Antibody (Catalog # MAB4481, filled histo­gram) or isotype control antibody (Catalog # MAB006, open histo­gram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Recombinant Mouse IL‑17 (Catalog # 421-ML) induces IL-6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line), as measured by the Mouse IL-6 Quantikine kit (Catalog # M6000B). IL-6 secretion elicited by Recombinant Mouse IL‑17 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑17 RA/IL‑17 R Monoclonal Antibody (Catalog # MAB4481). The ND50 is typically 0.2‑0.8 μg/mL.

IL-17RA is an alternative and better target than IL-25. (A) IL-17 ligand and receptor family. IL-17A, IL-17A/F, IL-17F, IL-17E (IL-25), IL-17B, IL-17C, and IL-17D are either homodimers or heterodimers. (B) Schematic representation of the experimental design for administering anti-IL-17RA antibody (100 ug) twice in combination with aPD-1+aCTLA-4. (Ci) Tumor growth curve of aIL-17RA treated mice compared with those without anti-IL-17RA treatment. (Cii) Average tumor volumes on day 18. (D) Gradings of immune infiltration on day 25. (E) Flow cytometry plot of percent IL-17RA+ in CD4+ T cell and CD8+ T cell from Lpr mice splenocytes (T cells—CD3+ gate). (F) Flow cytometry analysis of CD69 activation marker expression in IL-17RA+ and IL-17RA− CD4+ T cells. Flow cytometry analysis of IL-17RA in central and effector CD4 T cells. (Gi) Exhausted (PD1+ CD69-) CD4+ T cells presented in mice of previous in vivo experiments. (Gii) Sample exhausted cells gating of representative mice from aPD-1/aCTLA-4 and aPD-1/aCTLA-4/aIL-17RA treatment groups. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38519059), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: Flow, B/N, CyTOF-ready
• Clone: 657603
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-17RA/IL-17R Antibody (657603) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IL‑17 RA/IL‑17 R
  Ser32-Trp322
  Accession # Q60943
• Specificity: Detects mouse IL‑17 RA/IL‑17 R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human IL-17 RA, recombinant mouse (rm) IL-17B R, rmIL-17 RD, or rmIL-17 RC is observed. In Western blots, less than 10% cross‑reactivity with recombinant human (rh) IL-17 RA or rhIL-17B R is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: IL17RA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 0.2-0.8 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17RA/IL-17R Antibody (657603)

• CD217 antigen
• CD217
• Cdw217
• CDw217interleukin 17 receptor
• hIL-17R
• IL-17 R
• IL-17 RA
• IL-17 receptor A
• IL17RA
• IL-17RA
• IL-17RAMGC10262
• IL17Rinterleukin-17 receptor A
• interleukin 17 receptor A

Background

IL-17 receptor alpha subunit (IL-17 RA), also known as IL-17 R, is a widely expressed 120 kDa glycosylated type I transmembrane protein that plays a central role in inflammatory responses. IL‑17 RA associates with IL-17 RC to form a signaling receptor complex for IL-17 and IL-17F. IL-17 RA ligation promotes T cell activation and the production of IL-6, G‑CSF, SCF, and multiple proinflammatory chemokines. Within the ECD, mouse IL-17 RA shares 84% and 72% aa sequence identity with rat and human IL17 RA, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-17RA/IL-17R Antibody (MAB4481)(11)

Publications using MAB4481

• Wang, X;Bao, H;Wang, Y;Wang, Y;Guo, C;Wu, Y;Xu, Y;Li, Y; Innovative peptide therapeutics targeting IL17RA to regulate inflammatory responses Scientific reports 2025-03-22 [PMID: 40121226] (Neutralization, Human)
• Li, T;Hu, L;Qin, C;Li, Y;Song, Z;Jiao, Y;Wang, C;Cui, W;Zhang, L; Annexin 1 Reduces Dermatitis-Induced Itch and Cholestatic Itch through Inhibiting Neuroinflammation and Iron Overload in the Spinal Dorsal Horn of Mice Brain sciences 2024-04-28 [PMID: 38790419] (In vivo assay, Mouse)
• Lv, XJ;Lv, SS;Wang, GH;Chang, Y;Cai, YQ;Liu, HZ;Xu, GZ;Xu, WD;Zhang, YQ; Glia-derived adenosine in the ventral hippocampus drives pain-related anxiodepression in a mouse model resembling trigeminal neuralgia Brain, behavior, and immunity 2024-01-18 [PMID: 38244946] (Immunohistochemistry, Neutralization, Mouse)
• D Knizkova, M Pribikova, H Draberova, T Semberova, T Trivic, A Synackova, A Ujevic, J Stefanovic, A Drobek, M Huranova, V Niederlova, O Tsyklauri, A Neuwirth, J Tureckova, O Stepanek, P Draber CMTM4 is a subunit of the IL-17 receptor and mediates autoimmune pathology Nature Immunology, 2022-10-21;0(0):. 2022-10-21 [PMID: 36271145] (Flow Cytometry, Mouse)
• T Yoshida, NA Das, AJ Carpenter, R Izadpanah, SA Kumar, S Gautam, SB Bender, U Siebenlist, B Chandrasek Minocycline reverses IL-17A/TRAF3IP2-mediated p38 MAPK/NF-kappaB/iNOS/NO-dependent cardiomyocyte contractile depression and death Cell. Signal., 2020-06-15;73(0):109690. 2020-06-15 [PMID: 32553549] (Neutralization, Western Blot, Mouse)
• Mahmoud M. Saleh, Alyse L. Frisbee, Jhansi L. Leslie, Erica L. Buonomo, Carrie A. Cowardin, Jennie Z. Ma et al. Colitis-Induced Th17 Cells Increase the Risk for Severe Subsequent Clostridium difficile Infection Cell Host & Microbe 2019-05-08 [PMID: 31003940]
• J Yan, Y Li, H Yang, L Zhang, B Yang, M Wang, Q Li Interleukin-17A participates in podocyte injury by inducing IL-1? secretion through ROS-NLRP3 inflammasome-caspase-1 pathway Scand. J. Immunol., 2018-03-05;0(0):. 2018-03-05 [PMID: 29446486] (Neutralization, Mouse)
• EA Akbay, S Koyama, Y Liu, R Dries, LE Bufe, M Silkes, MM Alam, DM Magee, R Jones, M Jinushi, M Kulkarni, J Carretero, X Wang, T Warner-Hat, JD Cavanaugh, A Osa, A Kumanogoh, GJ Freeman, MM Awad, DC Christiani, R Bueno, PS Hammerman, G Dranoff, KK Wong Interleukin-17A Promotes Lung Tumor Progression Through Neutrophil Attraction to Tumor Sites and Mediating Resistance to PD-1 Blockade J Thorac Oncol, 2017-05-06;0(0):. 2017-05-06 [PMID: 28483607] (Neutralization, Mouse)
• AW Qiu, QH Liu, JL Wang Blocking IL-17A Alleviates Diabetic Retinopathy in Rodents Cell. Physiol. Biochem, 2017-02-21;41(3):960-972. 2017-02-21 [PMID: 28222445] (Neutralization, Mouse, Rat)
• Keratinocytes contribute intrinsically to psoriasis upon loss of Tnip1 function Proc Natl Acad Sci USA, 2016-09-26;0(0):. 2016-09-26 [PMID: 27671649] (Neutralization, Mouse)
• Wu S, Yu J, Liu F, Miaw S, Wu-Hsieh B Galectin-3 negatively regulates dendritic cell production of IL-23/IL-17-axis cytokines in infection by Histoplasma capsulatum. J Immunol, 2013-03-01;190(7):3427-37. 2013-03-01 [PMID: 23455499] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: IL17RA
• Uniprot:
  • Q60943()