IL-17RA/IL-17R Antibody (49M4D2) - BSA Free

Western Blot: IL-17 RA/IL-17 R Antibody (49M4D2) [NBP2-25258] - Analysis of an IL-17RA recombinant protein fragment and human heart lysate using IL-17RA antibody at 0.5 and 3 ug/ml, respectively. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: IL-17 RA/IL-17 R Antibody (49M4D2) [NBP2-25258] - Formalin-fixed, paraffin-embedded human spleen stained with IL-17RA antibody (1 ug/ml), peroxidase-conjugate and DAB chromogen. TMA was used for this test.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 49M4D2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

IL-17RA/IL-17R Antibody (49M4D2) - BSA Free Summary

• Description: Novus Biologicals Mouse IL-17RA/IL-17R Antibody (49M4D2) - BSA Free (NBP2-25258) is a monoclonal antibody validated for use in IHC and WB. Anti-IL-17RA/IL-17R Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Within the range of amino acids 186-267 of human IL-17RA were used as the immunogen for the antibody.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL17RA
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20-1:1000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for IL-17RA/IL-17R Antibody (49M4D2) - BSA Free

• CD217 antigen
• CD217
• Cdw217
• CDw217interleukin 17 receptor
• hIL-17R
• IL-17 R
• IL-17 RA
• IL-17 receptor A
• IL17RA
• IL-17RA
• IL-17RAMGC10262
• IL17Rinterleukin-17 receptor A
• interleukin 17 receptor A

Background

IL-17RA (IL-17 receptor A), a type I transmembrane protein with a ubiquitous cell and tissue distribution has no homology with any known cytokine receptor family. This receptor is a member of the IL-17 Receptor family. IL-17RA encodes an unusually large cytoplasmic tail and was shown to activate inflammatory events typical of innate cytokines such as TNFa and IL-1b including activation of the transcription factor NF-KappaB. IL-17RA is highly expressed on hematopoietic cells, but also at lower levels on osteoblasts, fibroblasts, endothelial cells, and epithelial cells (Chang SH, 2011) (Zhu S, 2010).>The role of the IL-17 family during parasite infections is an emerging area of research with often contradicting results. In this regard, IL-17RA signaling has been shown to be both deleterious and protective during Toxoplasma gondii infection. Furthermore, two groups demonstrated that IL-17 plays a protective role in T. cruzi infection although the underlying mechanisms remains poorly understood. Indeed, both reports showed significant contradictions that might be related to the different experimental settings but deserves further investigation and discussion, since members of the IL-17 family, IL-17A, IL-17E and IL-17F, are produced during T. cruzi infection. Furthermore, it is confirmed that IL-17RA signaling is required for host resistance during the T. cruzi infection and focused at the mechanisms underlying IL-17RA-mediated protective effect. IL-17RA antibody has the potential to be a therapeutic option for many diseases (Raychaudhuri SP, 2012) (Tosello Boari J .2012).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-17RA/IL-17R Antibody (NBP2-25258)(5)

Publications using NBP2-25258

• Kie?b P, Kaczorowski M, Kowalczyk K et al. Role of IL-17A and IL-17RA in Prostate Cancer with Lymph Nodes Metastasis: Expression Patterns and Clinical Significance Cancers (Basel) 2023-09-15 [PMID: 37760548] (Human)
• Lo CY, Wang CH, Wang CW et al. Increased Interleukin-17 and Glucocorticoid Receptor-beta Expression in Interstitial Lung Diseases and Corticosteroid Insensitivity Frontiers in immunology 2022-07-05 [PMID: 35865549] (IHC-P, Human)
• de Barros De Luccia T, Ono E, Menon R et al. The effect of Gestational Diabetes Mellitus on the fetal compartment Journal of Reproductive Immunology 2021-03-01 [PMID: 33836321] (IHC-P, Human)
• Zhang J, Wang D, Wang L et al. Pro-fibrotic effects of IL-17A and elevated IL-17RA in IPF and RA-ILD support a direct role for IL-17A/IL-17RA in human fibrotic interstitial lung disease. Am. J. Physiol. Lung Cell Mol. Physiol. 2019-01-03 [PMID: 30604628] (WB, Human)
• Alphy AS, Kannan TP, Norazmi MN, Nurul AA. Interleukin-17A promotes osteogenic differentiation by increasing OPG/RANKL ratio in stem cells from human exfoliated deciduous teeth. J Tissue Eng Regen Med 2018-05-18 [PMID: 29774992] (Human)
  
  Details:
  This citation used the Azide Free version of this antibody.

Bioinformatics

• Gene Symbol: IL17RA
• Uniprot:
  • NP_055154()