Human IL-17A/F Heterodimer ELISA Kit (Colorimetric)

ELISA: Human IL-17AF ELISA Kit [NBP2-31047] - Standard curve using the human IL-17AF standard.

Product Discontinued

Human IL-17A/F Heterodimer ELISA Kit (Colorimetric) Summary

• Specificity: Measures free human IL-17AF in the supernatant of activated cells.
• Standard Curve Range: 62.5-4000 pg/ml
• Sensitivity: 62.5 pg/ml
• Assay Type: Sandwich ELISA
• Sample Volume: 0.1 ml
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL17A

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: For the detection of human IL-17AF in the supernatant. The human IL-17AF ActivELISA(TM) Kit measures free IL-17AF in the supernatant of activated cells. Standard protocols for detecting IL-17AF activity include ELISA and Western Blot. The IL-17AF ActivELISA(TM) can be completed in one day using a sandwich ELISA protocol. The anti-IL-17F antibody is coated on a plate which then captures free IL-17AF. The amount of bound IL-17AF is detected by adding a biotinylated anti-IL-17A antibody followed by HRP-conjugated streptavidin. ABTS substrate is then added and the concentration is determined by colorimetric detection in an ELISA plate reader.

Packaging, Storage & Formulations

• Storage: Storage is content dependent.

Notes

Additional items required: Distilled water 96-well ELISA plate reader

Alternate Names for Human IL-17A/F Heterodimer ELISA Kit (Colorimetric)

• CTLA8
• CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
• Cytotoxic T-lymphocyte-associated antigen 8
• Cytotoxic T-Lymphocyte-Associated Protein 8
• IL17
• IL-17
• IL-17A
• IL-17A/F Heterodimer
• interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
• interleukin 17A
• interleukin-17A

Background

Interleukin 17 (IL-17) is a pro-inflammatory cytokine produced by a subset of T helper cells that develops distinct from the Th1- and Th2- cell differentiation pathways. IL-17, also known as CTLA-8, stimulates induction of other pro-inflammatory cytokines TNF alpha, IL-1 beta, IL-6, and IL-8, and reports strongly suggest the involvement of IL-17 in several chronic inflammatory diseases such as rheumatoid arthritis, psoriasis and multiple sclerosis. TGFB (differentiation) and IL-23 (expansion) are required for induction and maintenance of Th17 (IL-17 producing) cells, which in turn induce the other proinflammatory cytokines. IL-17 (~32kDa) protein is produced and exists as a homo-dimer, has homology to a herpes virus early protein, is one of the six members (IL-17A-F) of this cytokine family, and is well characterized and highly expressed by activated effector memory T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for IL-17A/F Heterodimer ELISA Kit (NBP2-31047). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL17A