Human IL-17A/F Heterodimer ELISA Kit (Colorimetric)

ELISA: Human IL-17AF ELISA Kit [NBP2-31047] - Standard curve using the human IL-17AF standard.

Product Discontinued

Human IL-17A/F Heterodimer ELISA Kit (Colorimetric) Summary

• Specificity: Measures free human IL-17AF in the supernatant of activated cells.
• Standard Curve Range: 62.5-4000 pg/ml
• Sensitivity: 62.5 pg/ml
• Assay Type: Sandwich ELISA
• Sample Volume: 0.1 ml
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL17A

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: For the detection of human IL-17AF in the supernatant. The human IL-17AF ActivELISA(TM) Kit measures free IL-17AF in the supernatant of activated cells. Standard protocols for detecting IL-17AF activity include ELISA and Western Blot. The IL-17AF ActivELISA(TM) can be completed in one day using a sandwich ELISA protocol. The anti-IL-17F antibody is coated on a plate which then captures free IL-17AF. The amount of bound IL-17AF is detected by adding a biotinylated anti-IL-17A antibody followed by HRP-conjugated streptavidin. ABTS substrate is then added and the concentration is determined by colorimetric detection in an ELISA plate reader.

Packaging, Storage & Formulations

• Storage: Storage is content dependent.

Notes

Additional items required: Distilled water 96-well ELISA plate reader

Alternate Names for Human IL-17A/F Heterodimer ELISA Kit (Colorimetric)

• CTLA8
• CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
• Cytotoxic T-lymphocyte-associated antigen 8
• Cytotoxic T-Lymphocyte-Associated Protein 8
• IL17
• IL-17
• IL-17A
• IL-17A/F Heterodimer
• interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
• interleukin 17A
• interleukin-17A

Background

Interleukin 17 (IL-17) is a pro-inflammatory cytokine produced by a subset of T helper cells that develops distinct from the Th1- and Th2- cell differentiation pathways. IL-17, also known as CTLA-8, stimulates induction of other pro-inflammatory cytokines TNF alpha, IL-1 beta, IL-6, and IL-8, and reports strongly suggest the involvement of IL-17 in several chronic inflammatory diseases such as rheumatoid arthritis, psoriasis and multiple sclerosis. TGFB (differentiation) and IL-23 (expansion) are required for induction and maintenance of Th17 (IL-17 producing) cells, which in turn induce the other proinflammatory cytokines. IL-17 (~32kDa) protein is produced and exists as a homo-dimer, has homology to a herpes virus early protein, is one of the six members (IL-17A-F) of this cytokine family, and is well characterized and highly expressed by activated effector memory T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for IL-17A/F Heterodimer ELISA Kit (NBP2-31047). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: IL17A