IL-17/IL-17A Antibody (905521R) [Alexa Fluor™ Plus 594]

• Reactivity: Rt
• Clone: 905521R
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 594

IL-17/IL-17A Antibody (905521R) [Alexa Fluor™ Plus 594] Summary

• Specificity: Detects Rat IL‑17/IL‑17A in direct and capture ELISAs. In direct Elisa, no cross-reactivity with Rat IL17F was observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17/IL-17A Antibody (905521R) [Alexa Fluor™ Plus 594]

• CTLA8
• CTLA-8
• CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
• Cytotoxic T-lymphocyte-associated antigen 8
• IL17
• IL-17
• IL17A
• IL-17A
• IL-17Acytotoxic T-lymphocyte-associated protein 8
• IL-17CTLA-8
• IL17interleukin-17A
• interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
• interleukin 17A

Background

Rat IL-17A/F is an approximately 37 kDa, secreted, disulfide-linked glycosylated heterodimeric protein comprised of two members of the IL-17 family of cytokines, IL-17A and IL-17F (1, 2). Members of this family demonstrate a structural motif termed a cysteine knot that also characterizes a large superfamily of growth factors such as TGF-beta (1). Although most cysteine knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links (1, 3-5). Mature rat IL-17A and IL-17F share 60% and 59% amino acid sequence identity with human and 61% and 90% with mouse IL-17A and IL-17F, respectively. They also share 50% aa sequence identity with each other (1). IL-17A/F heterodimer and the IL-17A and IL-17F homodimers are produced by IL-23 activated Th17 cells (1, 6-10, 14). The widely expressed receptors IL-17 RA and IL-17 RC form a heterodimer for the binding of
IL-17A and IL-17F, as well as the heterodimeric IL-17A/F (6, 11, 12). IL-17A/F is a biologically active protein that induces chemokine production and airway neutrophilia with intermediate potency between IL-17A (most potent) and IL-17F (least potent) (7, 12). It is up-regulated in immune cells during inflammatory arthritis and contributes to disease severity (13).

1. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
2. Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
3. Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
4. Kolls, J.K. and A. Linden (2004) Immunity 21:467.
5. Gu, C. et al. (2013) Cytokine 64:477.
6. Cheung, P.F.Y. et al. (2008) J. Immunol. 180:5625.
7. Liang, S.C. et al. (2007) J. Immunol. 179:7791.
8. Ouyang, W. et al. (2008) Immunity 28:454.
9. Steinman, L. (2007) Nat. Med. 13:139.
10. Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
11. Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
12. Wright, J.F. et al. (2008) J. Immunol. 181:2799.
13. Sarkar, S. et al. (2014) Clin. Exp. Immunol. 177:652.
14. Spolski, R. et al. (2009) Eur. J. Immunol. 3: 658.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for IL-17/IL-17A Antibody (FAB8410AFP594). (Showing 1 - 2 of 2 FAQ).

1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
   • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
2. I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
   • Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).