IL-17/IL-17A Antibody (881309) [Phycoerythrin]

Mouse splenocytes stimulated to induce Th17 cells were stained with Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A) and either (A) Rat Anti-Mouse IL-17/IL-17A PE-conjugated Monoclonal Antibody (Catalog # IC7211P) or (B) Rat IgG2BPhycoerythrin Isotype Control (Catalog # IC013P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

• Reactivity: Mu
• Applications: Flow
• Clone: 881309
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Phycoerythrin

IL-17/IL-17A Antibody (881309) [Phycoerythrin] Summary

• Immunogen: E. coli-derived recombinant mouse IL‑17/IL‑17A
  Thr22-Ala158
  Accession # Q62386
• Specificity: Detects mouse IL‑17/IL‑17A in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17/IL-17A Antibody (881309) [Phycoerythrin]

• CTLA8
• CTLA-8
• CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
• Cytotoxic T-lymphocyte-associated antigen 8
• IL17
• IL-17
• IL17A
• IL-17A
• IL-17Acytotoxic T-lymphocyte-associated protein 8
• IL-17CTLA-8
• IL17interleukin-17A
• interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
• interleukin 17A

Background

Interleukin 17 (IL-17), also known as IL-17A and CTLA-8, is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Mouse IL-17 cDNA encodes a 158 amino acid (aa) residue precursor protein with a 26 amino acid residue signal peptide that is cleaved to yield the 132 aa residue mature  IL-17. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers and IL-17 is typically found as a heterodimer with IL-17F. At the amino acid level, mouse IL-17 shows 57%, 61%, and 87% sequence identity with herpes virus, human, and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has been cloned. While the expression of IL-17 mRNA is restricted to activated alpha beta TCR⁺CD4^-CD8^- T cells, the expression of mouse IL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 has multiple biological effects on a variety of cells including the induction of IL-6 and IL-8 production by fibroblasts, the enhancement of surface expression of ICAM-1 on fibroblasts, and the activation of NF-kappa B and costimulation of proliferation by T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for IL-17/IL-17A Antibody (IC7211P). (Showing 1 - 2 of 2 FAQ).

1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
   • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
2. I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
   • Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

Bioinformatics

• Uniprot:
  • Q62386()