IL-17/IL-17A Antibody (41809) [Unconjugated]

Images

 
IL‑17/IL‑17A was detected in immersion fixed paraffin-embedded sections of human Crohn's disease intestine using Mouse Anti-Human/Primate IL‑17/IL‑17A Monoclonal Antibody (Catalog # MAB317) at 5 µg/mL ...read more
Human IL-17 was immunoprecipitated from 100 μg of human primary differentiated Th17 cell lysate following incubation with 3 µg Mouse Anti-Human/Primate IL-17 Monoclonal Antibody (Catalog # MAB317) or isotype control ...read more
Recombinant Human IL‑17 (Catalog # 317-ILB) stimulates IL‑6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line), as measured by the Mouse IL‑6 Quantikine ELISA ...read more

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications IHC, IP, B/N, ELISA(Cap)
Clone
41809
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
View Available Formulations

IL-17/IL-17A Antibody (41809) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IL-17
Ile20-Ala155
Accession # Q16552
Specificity
Detects human and primate IL-17 in direct ELISAs. In direct ELISAs, approximately 12% cross-reactivity with recombinant canine IL-17 is observed and 25%-50% reactivity with recombinant human (rh) IL-17A/IL-17F heterodimer is observed. No cross-reactivity with recombinant mouse IL-17, rhIL-17B, rhIL-17C, rhIL-17D, rhIL-17E, or rhIL-17F is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
IL17A
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-25 ug/mL
  • Immunoprecipitation 3 ug/100 ug cell lysate
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Neutralization 1-3 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Detection: Human IL-17 Biotinylated Antibody (Catalog number BAF317)
Standard: Recombinant Human IL-17 (Catalog number 317-ILB)
Publications
Read Publications using
MAB317 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17/IL-17A Antibody (41809) [Unconjugated]

  • CTLA8
  • CTLA-8
  • CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
  • Cytotoxic T-lymphocyte-associated antigen 8
  • IL17
  • IL-17
  • IL17A
  • IL-17A
  • IL-17Acytotoxic T-lymphocyte-associated protein 8
  • IL-17CTLA-8
  • IL17interleukin-17A
  • interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
  • interleukin 17A

Background

IL-17 is a pro-inflammatory cytokine secreted by activated T cells. It is the prototype member of the IL-17 family that also includes IL-17B, C, D, E, and F.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
MAB15041
Species: Hu
Applications: WB, IHC, CyTOF-ready, ICFlow
NB100-39002
Species: Hu, Mu, Rt, Bv
Applications: WB, Flow, IB, ICC/IF, IHC, IHC-P, IP, Single-Cell Western
NBP2-27327
Species: Hu
Applications: WB, IHC, IHC-P
MAB177
Species: Hu
Applications: WB, Flow, CyTOF-ready, KO, Neut
NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
MAB317
Species: Hu, Pm
Applications: IHC, IP, B/N, ELISA(Cap)

Publications for IL-17/IL-17A Antibody (MAB317)(17)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 7 applications: ELISA Capture, ELISA Development, ELISA Development (Capture), Flow Cytometry, IHC-P, Luminex Development, Neutralization.


Filter By Application
ELISA Capture
(2)
ELISA Development
(3)
ELISA Development (Capture)
(3)
Flow Cytometry
(1)
IHC-P
(2)
Luminex Development
(1)
Neutralization
(5)
All Applications
Filter By Species
Human
(17)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using MAB317 Applications Species
JC Chamcheu, S Esnault, VM Adhami, AL Noll, S Banang-Mbe, T Roy, SS Singh, S Huang, KG Kousoulas, H Mukhtar Fisetin, a 3,7,3&#039;,4&#039;-Tetrahydroxyflavone Inhibits the PI3K/Akt/mTOR and MAPK Pathways and Ameliorates Psoriasis Pathology in 2D and 3D Organotypic Human Inflammatory Skin Models Cells, 2019;8(9):. 2019 [PMID: 31540162] (ELISA Capture, Human) ELISA Capture Human
J Jhun, SH Lee, SK Lee, HY Kim, ES Jung, DG Kim, J Choi, SH Bae, SK Yoon, BH Chung, CW Yang, ML Cho, JY Choi Serial Monitoring of Immune Markers Being Represented Regulatory T Cell/T Helper 17 Cell Ratio: Indicating Tolerance for Tapering Immunosuppression after Liver Transplantation Front Immunol, 2018;9(0):352. 2018 [PMID: 29545795] (ELISA Capture, Human) ELISA Capture Human
R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3 Cell. Physiol. Biochem., 2017;42(5):1961-1972. 2017 [PMID: 28793286] (ELISA Development (Capture), Human) ELISA Development (Capture) Human
E Brencicova, AL Jagger, HG Evans, M Georgouli, A Laios, S Attard Mon, G Mehra, J Spencer, AA Ahmed, S Raju-Kanki, LS Taams, SS Diebold Interleukin-10 and prostaglandin E2 have complementary but distinct suppressive effects on Toll-like receptor-mediated dendritic cell activation in ovarian carcinoma PLoS ONE, 2017;12(4):e0175712. 2017 [PMID: 28410380] (ELISA Development (Capture), Human) ELISA Development (Capture) Human
Dobrina N Hull Increase in circulating Th17 cells during anti-TNF therapy is associated with ultrasonographic improvement of synovitis in rheumatoid arthritis Arthritis Res. Ther, 2016;18(1):303. 2016 [PMID: 28010726] (ELISA Development (Capture), Human) ELISA Development (Capture) Human
Schminke B, Trautmann S, Mai B, Miosge N, Blaschke S Interleukin 17 inhibits progenitor cells in rheumatoid arthritis cartilage. Eur J Immunol, 2015;46(2):440-5. 2015 [PMID: 26558442] (Neutralization, Human) Neutralization Human
Todd J, Simpson P, Estis J, Torres V, Wub A Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays. Cytokine, 2013;64(3):660-5. 2013 [PMID: 24128872] (ELISA Development, Human) ELISA Development Human
Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH TH17 cytokines induce human airway smooth muscle cell migration. J. Allergy Clin. Immunol., 2011;127(4):1046-53.e1-2. 2011 [PMID: 21345484] (Neutralization, Human) Neutralization Human
Heo YJ, Joo YB, Oh HJ, Park MK, Heo YM, Cho ML, Kwok SK, Ju JH, Park KS, Cho SG, Park SH, Kim HY, Min JK IL-10 suppresses Th17 cells and promotes regulatory T cells in the CD4+ T cell population of rheumatoid arthritis patients. Immunol. Lett., 2010;127(2):150-6. 2010 [PMID: 19895848] (ELISA Development, Human) ELISA Development Human
Zrioual S, Ecochard R, Tournadre A, Lenief V, Cazalis MA, Miossec P Genome-wide comparison between IL-17A- and IL-17F-induced effects in human rheumatoid arthritis synoviocytes. J. Immunol., 2009;182(0):3112-20. 2009 [PMID: 19234208] (IHC-P, Human) IHC-P Human
Show All 17 Publications.

Reviews for IL-17/IL-17A Antibody (MAB317) (0)

There are no reviews for IL-17/IL-17A Antibody (MAB317). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IL-17/IL-17A Antibody (MAB317). (Showing 1 - 2 of 2 FAQs).

  1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
    • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
  2. I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
    • Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the following link to see the available fluors: http://www.novusbio.com/support/custom-antibody-labeling.html The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional IL-17/IL-17A Products

Bioinformatics Tool for IL-17/IL-17A Antibody (MAB317)

Discover related pathways, diseases and genes to IL-17/IL-17A Antibody (MAB317). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IL-17/IL-17A Antibody (MAB317)

Discover more about diseases related to IL-17/IL-17A Antibody (MAB317).
 

Pathways for IL-17/IL-17A Antibody (MAB317)

View related products by pathway.

PTMs for IL-17/IL-17A Antibody (MAB317)

Learn more about PTMs related to IL-17/IL-17A Antibody (MAB317).

Blogs on IL-17/IL-17A

There are no specific blogs for IL-17/IL-17A, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our IL-17/IL-17A Antibody (41809) [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol IL17A
Uniprot