IL-15R alpha Antibody [Biotin]

• Reactivity: Mu
• Applications: WB, Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

IL-15R alpha Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IL-15 R alpha
  Gly33-Lys205
  Accession # Q60819
• Specificity: Detects mouse IL-15 R alpha in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) IL‑15 R alpha is observed and less than 1% cross-reactivity with rhIL-2 R alpha , recombinant mouse (rm) IL-2 R beta , and rmIL-2 R gamma is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL15RA
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-15R alpha Antibody [Biotin]

• CD215 antigen
• CD215
• IL15 R alpha
• IL-15 R alpha
• IL-15 receptor subunit alpha
• IL-15R alpha
• IL15RA
• IL-15Ra
• IL-15R-alpha
• interleukin 15 receptor, alpha
• interleukin-15 receptor subunit alpha
• MGC104179

Background

Interleukin 15 receptor alpha (IL-15 R alpha ) is a high affinity receptor that specifically binds IL-15 with high affinity and associates as a heterotrimer with the IL-2 receptors beta and gamma subunits to initiate signal transduction. IL-15 R alpha is expressed on a wide variety of T cells and B cells as well as non-lymphoid cells. IL‑15 R alpha is a 58-60 kDa protein that shares structural similarities to the IL-2 R alpha protein. IL-15 R alpha and IL-2 R alpha genes also share similar intron-exon organization and are closely linked on human chromosome 10p14-p15. Human IL-15 R alpha shares 45% amino acid (aa) homology with the mouse form of the receptor. Signaling of IL‑15 can occur in one of three ways; through the heterotrimeric complex of IL-15 R alpha , IL-2 R beta , and IL-2 R gamma c, through the heterodimeric complex of IL-2 receptors beta and gamma common, through a novel 60-65 kDa IL-15 RX subunit found on mast cells. The binding of IL-15 to IL-15 R alpha has been reported to antagonize the TNF‑ alpha -mediated apoptosis in fibroblasts by competing with TNF RI for TRAF2 binding.

1. Anderson, D.M. et al. (1995) J. Biol. Chem. 270:29862.
2. Bulfone-Paus, S. et al. (1999) FASEB 13:1575.
3. Waldemann, T.A. and Y. Tagaya (1999) Ann. Rev. Immunol. 17:19.
4. Dubois, S. et al. (1999) J. Biol. Chem. 274:26978.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-15R alpha Antibody (BAF551)(24)

Publications using BAF551

• Christopher J. Nicolai, Natalie Wolf, I-Chang Chang, Georgia Kirn, Assaf Marcus, Chudi O. Ndubaku et al. NK cells mediate clearance of CD8+ T cell-resistant tumors in response to STING agonists Science Immunology 3/20/2020 [PMID: 32198222]
• N Simonovi?, A Witalisz-S, K Meissl, C Lassnig, U Reichart, T Kolbe, M Farlik, C Bock, V Sexl, M Müller, B Strobl NK Cells Require Cell-Extrinsic and -Intrinsic TYK2 for Full Functionality in Tumor Surveillance and Antibacterial Immunity J. Immunol., 2019-02-04;0(0):. 2019-02-04 [PMID: 30718299] (Flow Cytometry, Mouse)
• Daniel J. Neitzke, Jacob S. Bowers, Kristina Andrijauskaite, Nathaniel S. O’Connell, Elizabeth Garrett-Mayer, John M. Wrangle et al. Murine Th17 cells utilize IL-2 receptor gamma chain cytokines but are resistant to cytokine withdrawal-induced apoptosis Cancer Immunology, Immunotherapy 6/1/2017 [PMID: 28280853]
• IL-15 signaling promotes adoptive effector T-cell survival and memory formation in irradiation-induced lymphopenia Cell Biosci, 2016-05-06;6(0):30. 2016-05-06 [PMID: 27158441] (Flow Cytometry, Mouse)
• Colpitts S, Puddington L, Lefrancois L IL-15 receptor alpha signaling constrains the development of IL-17-producing gammadelta T cells. Proc Natl Acad Sci U S A, 2015-07-20;112(31):9692-7. 2015-07-20 [PMID: 26195801] (Flow Cytometry, Mouse)
• Daniel J Puleston, Hanlin Zhang, Timothy J Powell, Elina Lipina, Stuart Sims, Isabel Panse et al. Autophagy is a critical regulator of memory CD8(+) T cell formation eLife 11/11/2014 [PMID: 25385531]
• Smita S. Iyer, Janel Hart Chatraw, Wendy G. Tan, E. John Wherry, Todd C. Becker, Rafi Ahmed et al. 1Protein Energy Malnutrition Impairs Homeostatic Proliferation of Memory CD8 T cells The Journal of Immunology 1/1/2012 [PMID: 22116826]
• Lee GA, Liou YH, Wang SW, Ko KL, Jiang ST, Liao NS Different NK cell developmental events require different levels of IL-15 trans-presentation. J. Immunol., 2011-06-29;187(3):1212-21. 2011-06-29 [PMID: 21715685] (Flow Cytometry, Mouse)
• Sato N, Sabzevari H, Fu S, Ju W, Petrus MN, Bamford RN, Waldmann TA, Tagaya Y Development of an IL-15-autocrine CD8 T-cell leukemia in IL-15-transgenic mice requires the cis expression of IL-15R alpha. Blood, 2011-02-08;117(15):4032-40. 2011-02-08 [PMID: 21304101] (Flow Cytometry, Mouse)
• Scott N. Mueller, William A. Langley, Guimei Li, Adolfo García-Sastre, Richard J. Webby, Rafi Ahmed Qualitatively Different Memory CD8+ T Cells Are Generated after Lymphocytic Choriomeningitis Virus and Influenza Virus Infections The Journal of Immunology 8/15/2010 [PMID: 20639484]
• Eidenschenk C, Crozat K, Krebs P, Arens R, Popkin D, Arnold CN, Blasius AL, Benedict CA, Moresco EM, Xia Y, Beutler B Flt3 permits survival during infection by rendering dendritic cells competent to activate NK cells. Proc. Natl. Acad. Sci. U.S.A., 2010-05-10;107(21):9759-64. 5/10/2010 [PMID: 20457904]
• Simone Haeberlein, Heidi Sebald, Christian Bogdan, Ulrike Schleicher IL-18, but not IL-15, contributes to the IL-12-dependent induction of NK-cell effector functions by Leishmania infantum in vivo European Journal of Immunology 3/10/2010 [PMID: 20213736]
• Sikora AG, Jaffarzad N, Hailemichael Y, Gelbard A, Stonier SW, Schluns KS, Frasca L, Lou Y, Liu C, Andersson HA, Hwu P, Overwijk WW IFN-alpha enhances peptide vaccine-induced CD8+ T cell numbers, effector function, and antitumor activity. J. Immunol., 2009-06-15;182(12):7398-407. 2009-06-15 [PMID: 19494262] (Flow Cytometry, Mouse)
• Zhang M, Yao Z, Dubois S, Ju W, Muller JR, Waldmann TA Interleukin-15 combined with an anti-CD40 antibody provides enhanced therapeutic efficacy for murine models of colon cancer. Proc. Natl. Acad. Sci. U.S.A., 2009-04-21;106(18):7513-8. 2009-04-21 [PMID: 19383782] (Flow Cytometry, Mouse)
• Rowley J, Monie A, Hung CF, Wu TC Inhibition of tumor growth by NK1.1+ cells and CD8+ T cells activated by IL-15 through receptor beta/common gamma signaling in trans. J. Immunol., 2008-12-15;181(12):8237-47. 2008-12-15 [PMID: 19050240] (Flow Cytometry, Mouse)
• Vang KB, Yang J, Mahmud SA, Burchill MA, Vegoe AL, Farrar MA IL-2, -7, and -15, but not thymic stromal lymphopoeitin, redundantly govern CD4+Foxp3+ regulatory T cell development. J. Immunol., 2008-09-01;181(5):3285-90. 2008-09-01 [PMID: 18714000] (Flow Cytometry, Mouse)
• Rubinstein MP, Lind NA, Purton JF, Filippou P, Best JA, McGhee PA, Surh CD, Goldrath AW IL-7 and IL-15 differentially regulate CD8+ T-cell subsets during contraction of the immune response. Blood, 2008-08-08;112(9):3704-12. 2008-08-08 [PMID: 18689546] (Flow Cytometry, Mouse)
• Sabatos CA, Doh J, Chakravarti S, Friedman RS, Pandurangi PG, Tooley AJ, Krummel MF A synaptic basis for paracrine interleukin-2 signaling during homotypic T cell interaction. Immunity, 2008-07-31;29(2):238-48. 2008-07-31 [PMID: 18674934] (ICC, Mouse)
• Mortier E, Woo T, Advincula R, Gozalo S, Ma A IL-15Ralpha chaperones IL-15 to stable dendritic cell membrane complexes that activate NK cells via trans presentation. J. Exp. Med., 2008-05-05;205(5):1213-25. 5/5/2008 [PMID: 18458113]
• Terme M, Chaput N, Combadiere B, Ma A, Ohteki T, Zitvogel L Regulatory T cells control dendritic cell/NK cell cross-talk in lymph nodes at the steady state by inhibiting CD4+ self-reactive T cells. J. Immunol., 2008-04-01;180(7):4679-86. 2008-04-01 [PMID: 18354191] (Flow Cytometry, Mouse)
• Lai YG, Hou MS, Hsu YW, Chang CL, Liou YH, Tsai MH, Lee F, Liao NS IL-15 does not affect IEL development in the thymus but regulates homeostasis of putative precursors and mature CD8 alpha alpha+ IELs in the intestine. J. Immunol., 2008-03-15;180(6):3757-65. 2008-03-15 [PMID: 18322181] (Flow Cytometry, Mouse)
• Hammerbeck CD, Mescher MF Antigen controls IL-7Ralpha expression levels on CD8 T cells during full activation or tolerance induction. J. Immunol., 2008-02-15;180(4):2107-16. 2008-02-15 [PMID: 18250416] (Functional Assay, Mouse)
• French AR, Sjolin H, Kim S, Koka R, Yang L, Young DA, Cerboni C, Tomasello E, Ma A, Vivier E, Karre K, Yokoyama WM DAP12 signaling directly augments proproliferative cytokine stimulation of NK cells during viral infections. J. Immunol., 2006-10-15;177(8):4981-90. 2006-10-15 [PMID: 17015680] (Flow Cytometry, Mouse)
• Gould MP, Greene JA, Bhoj V, DeVecchio JL, Heinzel FP Distinct modulatory effects of LPS and CpG on IL-18-dependent IFN-gamma synthesis. J. Immunol., 2004-02-01;172(3):1754-62. 2004-02-01 [PMID: 14734758] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: IL15RA
• Uniprot:
  • Q60819()