NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL-15 Ra was detected in immersion fixed HDLM-2 human Hodgkin's lymphoma cell line using Goat Anti-Human IL-15 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF247) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

147-IR) inhibits Recombinant Human IL-15 (Catalog # 247-IL) induced proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-15 (2 ng/mL) activity elicited by Recombinant Human IL-15 Ra Fc Chimera (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-15 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF247). The ND₅₀ is typically 1-4 µg/mL." class="big_lightbox"> 147-IR) inhibits Recombinant Human IL-15 (Catalog # 247-IL) induced proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-15 (2 ng/mL) activity elicited by Recombinant Human IL-15 Ra Fc Chimera (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-15 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF247). The ND₅₀ is typically 1-4 µg/mL." class="big_thumb" />

AF247) at 3ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to exocrine glands. " class="big_lightbox"> AF247) at 3ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to exocrine glands. " class="big_thumb" />

F0108). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> F0108). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

247-IL) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑15 (0.8 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑15 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF551). The ND₅₀ is typically 0.3-1.5 µg/mL." class="big_lightbox"> 247-IL) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑15 (0.8 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑15 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF551). The ND₅₀ is typically 0.3-1.5 µg/mL." alt="Recombinant Human IL‑15 (Catalog # 247-IL) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑15 (0.8 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑15 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF551). The ND₅₀ is typically 0.3-1.5 µg/mL." class="big_thumb" />

415-ML) for 5 days, 1 µg/mL LPS on day 6 and harvested on day 7 were stained with Rat Anti-Mouse IL-15 Ra Monoclonal Antibody (Catalog # MAB5511, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B)." class="big_lightbox"> 415-ML) for 5 days, 1 µg/mL LPS on day 6 and harvested on day 7 were stained with Rat Anti-Mouse IL-15 Ra Monoclonal Antibody (Catalog # MAB5511, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B)." alt="C57BL/6 mouse bone marrow cells differentiated into dendritic cells by treatment with 20 ng/mL Recombinant Mouse GM-CSF (Catalog # 415-ML) for 5 days, 1 µg/mL LPS on day 6 and harvested on day 7 were stained with Rat Anti-Mouse IL-15 Ra Monoclonal Antibody (Catalog # MAB5511, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B)." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="IL-15R alpha was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human IL-15R alpha Monoclonal Antibody (Catalog # MAB1472) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

285-IF; 25 ng/ml) for 24 hours, then stained with (A) Rabbit Anti-Human IL-15R alpha Monoclonal Antibody (Catalog # MAB10900) or (B) Rabbit IgG Control (MAB1050), followed by APC-conjugated anti-Rabbit IgG secondary antibody (F0111) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (FAB3832P). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_lightbox"> 285-IF; 25 ng/ml) for 24 hours, then stained with (A) Rabbit Anti-Human IL-15R alpha Monoclonal Antibody (Catalog # MAB10900) or (B) Rabbit IgG Control (MAB1050), followed by APC-conjugated anti-Rabbit IgG secondary antibody (F0111) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (FAB3832P). Staining was performed using our Staining Membrane-associated Proteins protocol." alt="Human PBMC were treated with LPS (100 ng/ml) and recombinant human IFN gamma (285-IF; 25 ng/ml) for 24 hours, then stained with (A) Rabbit Anti-Human IL-15R alpha Monoclonal Antibody (Catalog # MAB10900) or (B) Rabbit IgG Control (MAB1050), followed by APC-conjugated anti-Rabbit IgG secondary antibody (F0111) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (FAB3832P). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_thumb" />

MAB1050), followed by APC-conjugated anti-Rabbit IgG secondary antibody (F0111) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (FAB3832P). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_lightbox"> MAB1050), followed by APC-conjugated anti-Rabbit IgG secondary antibody (F0111) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (FAB3832P). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_thumb" />