IL-15R alpha Antibody

Recombinant Human IL‑15 (Catalog # 247-IL) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑15 (0.8 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑15 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF551). The ND50 is typically 0.3-1.5 µg/mL.

• Reactivity: Mu
• Applications: WB, Flow, B/N, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-15R alpha Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IL-15 R alpha
  Gly33-Lys205
  Accession # Q60819
• Specificity: Detects mouse IL-15 R alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) IL‑15 R alpha is observed and less than 1% cross-reactivity with rhIL-2 R alpha , recombinant mouse (rm) IL-2 R beta , and rmIL-2 R gamma is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL15RA
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 0.3-1.5 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-15R alpha Antibody

• CD215 antigen
• CD215
• IL15 R alpha
• IL-15 R alpha
• IL-15 receptor subunit alpha
• IL-15R alpha
• IL15RA
• IL-15Ra
• IL-15R-alpha
• interleukin 15 receptor, alpha
• interleukin-15 receptor subunit alpha
• MGC104179

Background

Interleukin 15 receptor alpha (IL-15 R alpha ) is a high affinity receptor that specifically binds IL-15 with high affinity and associates as a heterotrimer with the IL-2 receptors beta and gamma subunits to initiate signal transduction. IL-15 R alpha is expressed on a wide variety of T cells and B cells as well as non-lymphoid cells. IL‑15 R alpha is a 58-60 kDa protein that shares structural similarities to the IL-2 R alpha protein. IL-15 R alpha and IL-2 R alpha genes also share similar intron-exon organization and are closely linked on human chromosome 10p14-p15. Human IL-15 R alpha shares 45% amino acid (aa) homology with the mouse form of the receptor. Signaling of IL-15 can occur in one of three ways; through the heterotrimeric complex of IL-15 R alpha , IL-2 R beta , and IL-2 R gamma c, through the heterodimeric complex of IL-2 receptors beta and gamma common, through a novel 60-65 kDa IL-15 RX subunit found on mast cells. The binding of IL-15 to IL-15 R alpha has been reported to antagonize the TNF-alpha -mediated apoptosis in fibroblasts by competing with TNF RI for TRAF2 binding.

1. Anderson, D.M. et al. (1995) J. Biol. Chem. 270:29862.
2. Bulfone-Paus, S. et al. (1999) FASEB 13:1575.
3. Waldemann, T.A. and Y. Tagaya (1999) Ann. Rev. Immunol. 17:19.
4. Dubois, S. et al. (1999) J. Biol. Chem. 274:26978.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-15R alpha Antibody (AF551)(8)

Publications using AF551

• Chiara Beilin, Kaushik Choudhuri, Gerben Bouma, Dessislava Malinova, Jaime Llodra, David L. Stokes et al. Dendritic cell-expressed common gamma-chain recruits IL-15 for trans-presentation at the murine immunological synapse Wellcome Open Research 2018-07-17 [PMID: 30483599] (Flow Cytometry, Neutralization, Mouse)
• Freeman B, Meyer C, Slifka M Anti-inflammatory cytokines directly inhibit innate but not adaptive CD8+ T cell functions. J Virol, 2014-04-16;88(13):7474-84. 2014-04-16 [PMID: 24741101] (Flow Cytometry, Mouse)
• Muller J, Waldmann T, Kruhlak M, Dubois S Paracrine and transpresentation functions of IL-15 are mediated by diverse splice versions of IL-15Ralpha in human monocytes and dendritic cells. J Biol Chem, 2012-10-16;287(48):40328-38. 2012-10-16 [PMID: 23074221] (Western Blot, Mouse)
• Klezovich-Benard M, Corre JP, Jusforgues-Saklani H, Fiole D, Burjek N, Tournier JN, Goossens PL Mechanisms of NK cell-macrophage Bacillus anthracis crosstalk: a balance between stimulation by spores and differential disruption by toxins. PLoS Pathog., 2012-01-12;8(1):e1002481. 2012-01-12 [PMID: 22253596] (Neutralization, Mouse)
• Yang CH, Tian L, Ling GS, Trendell-Smith NJ, Ma L, Lo CK, Stott DI, Liew FY, Huang FP Immunological mechanisms and clinical implications of regulatory T cell deficiency in a systemic autoimmune disorder: roles of IL-2 versus IL-15. Eur. J. Immunol., 2008-06-01;38(6):1664-76. 2008-06-01 [PMID: 18465774] (Flow Cytometry, Mouse)
• McCullar V, Oostendorp R, Panoskaltsis-Mortari A, Yun G, Lutz CT, Wagner JE, Miller JS Mouse fetal and embryonic liver cells differentiate human umbilical cord blood progenitors into CD56-negative natural killer cell precursors in the absence of interleukin-15. Exp. Hematol., 2008-03-04;36(5):598-608. 2008-03-04 [PMID: 18295962] (Flow Cytometry, Mouse)
• Chaudhry UI, Kingham TP, Plitas G, Katz SC, Raab JR, DeMatteo RP Combined stimulation with interleukin-18 and CpG induces murine natural killer dendritic cells to produce IFN-gamma and inhibit tumor growth. Cancer Res., 2006-11-01;66(21):10497-504. 2006-11-01 [PMID: 17079471] (Flow Cytometry, Mouse)
• Gould MP, Greene JA, Bhoj V, DeVecchio JL, Heinzel FP Distinct modulatory effects of LPS and CpG on IL-18-dependent IFN-gamma synthesis. J. Immunol., 2004-02-01;172(3):1754-62. 2004-02-01 [PMID: 14734758] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: IL15RA
• Uniprot:
  • Q60819()