IL-12/IL-35 p35 Antibody (27537) [Unconjugated]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, CyTOF-ready, Flow
Clone
27537
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

IL-12/IL-35 p35 Antibody (27537) [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑12/IL-35 p35
Arg23-Ser219
Accession # P29459
Specificity
Detects human IL‑12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL12A
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
Publications
Read Publications using
MAB1570 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-12/IL-35 p35 Antibody (27537) [Unconjugated]

  • IL-12/IL-35 p35
  • IL-12A
  • IL35p35

Background

Interleukin 12 (IL-12) and interleukin 35 (IL-35) are heterodimeric cytokines composed of alpha and beta chains. IL-12 is composed of p35 and p40 subunits, while IL-35 is comprised of p35 paired with EBI-3 (1). In mice, IL-35 is produced by FoxP3+ regulatory T cells and may function as an inhibitory cytokine to suppress T cell proliferation (2). Human FoxP3+ Tregs do not constitutively express IL-35 (3), but expression may be induced by activated dendritic cells (4).
  1. Collison, L.W. and D.A.A. Vignali (2008) Immunol. Rev. 226:248.
  2. Collison, L.W. et al. (2007) Nature 450:566.
  3. Bardel, E. et al. (2008) J. Immunol. 181:6898.
  4. Seyerl, M. et al.(2010) Eur. J. Immunol. 40:321.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-12/IL-35 p35 Antibody (MAB1570)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: Bioassay, ELISA Development, Flow Cytometry, Immunoprecipitation, Neutralization, Western Blot.


Filter By Application
Bioassay
(1)
ELISA Development
(1)
Flow Cytometry
(3)
Immunoprecipitation
(1)
Neutralization
(2)
Western Blot
(3)
All Applications
Filter By Species
Human
(8)
Mouse
(3)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using MAB1570 Applications Species
Li Wang Gene expression and antiviral activity of interleukin-35 in response to influenza A virus infection J Biol Chem, 2016;0(0):. 2016 [PMID: 27307042] (Western Blot, Human) Western Blot Human
MM Halpert, V Konduri, D Liang, Y Chen, JB Wing, S Paust, JM Levitt, WK Decker Dendritic Cell-Secreted Cytotoxic T-Lymphocyte-Associated Protein-4 Regulates the T-cell Response by Downmodulating Bystander Surface B7 Stem Cells Dev., 2016;25(10):774-87. 2016 [PMID: 26979751] (Western Blot, Human) Western Blot Human
Liu G, Bi Y, Xue L, Zhang Y, Yang H, Chen X, Lu Y, Zhang Z, Liu H, Wang X, Wang R, Chu Y, Yang R Dendritic cell SIRT1-HIF1alpha axis programs the differentiation of CD4+ T cells through IL-12 and TGF-beta1. Proc Natl Acad Sci U S A, 2015;112(9):E957-65. 2015 [PMID: 25730867] (Bioassay, Mouse) Bioassay Mouse
Hsiao H, Hsu T, Liu W, Hsieh W, Chou T, Wu Y, Jiang S, Lai M Deltex1 antagonizes HIF-1alpha and sustains the stability of regulatory T cells in vivo. Nat Commun, 2015;6(0):6353. 2015 [PMID: 25695215] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Rossetti M, Spreafico R, Saidin S, Chua C, Moshref M, Leong J, Tan Y, Thumboo J, van Loosdregt J, Albani S Ex vivo-expanded but not in vitro-induced human regulatory T cells are candidates for cell therapy in autoimmune diseases thanks to stable demethylation of the FOXP3 regulatory T cell-specific demethylated region. J Immunol, 2015;194(1):113-24. 2015 [PMID: 25452562] (Neutralization, Human) Neutralization Human
Kochetkova I, Thornburg T, Callis G, Holderness K, Maddaloni M, Pascual D Oral Escherichia coli colonization factor antigen I fimbriae ameliorate arthritis via IL-35, not IL-27. J Immunol, 2014;192(2):804-16. 2014 [PMID: 24337375] (ELISA Development, Mouse) ELISA Development Mouse
Olson B, Jankowska-Gan E, Becker J, Vignali D, Burlingham W, McNeel D Human prostate tumor antigen-specific CD8+ regulatory T cells are inhibited by CTLA-4 or IL-35 blockade. J Immunol, 2012;189(12):5590-601. 2012 [PMID: 23152566] (Neutralization, Human) Neutralization Human
Jindra C Human rhinoviruses induce IL-35-producing Treg via induction of B7-H1 (CD274) and sialoadhesin (CD169) on DC. Eur J Immunol, 2010;40(2):321-9. 2010 [PMID: 19950173] (Immunoprecipitation, Human) Immunoprecipitation Human
Maneechotesuwan K, Wamanuttajinda V, Kasetsinsombat K, Huabprasert S, Yaikwawong M, Barnes PJ, Wongkajornsilp A Der p 1 suppresses indoleamine 2, 3-dioxygenase in dendritic cells from house dust mite-sensitive patients with asthma. J. Allergy Clin. Immunol., 2009;123(1):239-48. 2009 [PMID: 19058839] (Flow Cytometry, Human) Flow Cytometry Human
Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells. Eur. J. Immunol., 2008;38(4):1012-23. 2008 [PMID: 18350544] (Flow Cytometry, Human) Flow Cytometry Human
Show All 11 Publications.

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Bioinformatics

Gene Symbol IL12A
Uniprot
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