S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑12/IL-35 p35 Arg23-Ser219 Accession # P29459
Detects human IL‑12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.5 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for IL-12/IL-35 p35 Antibody (27537) [Unconjugated]
12 (IL-12) and interleukin 35 (IL-35) are heterodimeric cytokines composed of alpha
and beta chains. IL-12 is composed of p35 and p40 subunits, while IL-35 is
comprised of p35 paired with EBI-3 (1). In mice, IL-35 is produced by FoxP3+
regulatory T cells and may function as an inhibitory cytokine to suppress T
cell proliferation (2). Human FoxP3+ Tregs do not constitutively
express IL-35 (3), but expression may be induced by activated dendritic cells
Collison, L.W. and D.A.A. Vignali (2008) Immunol. Rev. 226:248.
Collison, L.W. et al. (2007) Nature 450:566.
E. et al. (2008) J. Immunol. 181:6898.
Seyerl, M. et al.(2010) Eur. J. Immunol. 40:321.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for IL-12/IL-35 p35 Antibody (MAB1570)(11)
We have publications tested in 2 confirmed species: Human, Mouse.
We have publications tested in 6 applications: Bioassay, ELISA Development, Flow Cytometry, Immunoprecipitation, Neutralization, Western Blot.
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