IL-12/IL-35 p35 Antibody (27537) [DyLight 650]

• Reactivity: Hu, Mu
• Applications: WB, CyTOF-ready, Flow
• Clone: 27537
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 650

IL-12/IL-35 p35 Antibody (27537) [DyLight 650] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑12/IL-35 p35
  Arg23-Ser219
  Accession # P29459
• Specificity: Detects human IL‑12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL12A
• Purity: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product MAB1570. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from ascites

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for IL-12/IL-35 p35 Antibody (27537) [DyLight 650]

• CLMF p35
• CLMF
• cytotoxic lymphocyte maturation factor 1, p35
• Cytotoxic lymphocyte maturation factor 35 kDa subunit
• IL-12, subunit p35
• IL-12/IL-35 p35
• IL-12A
• IL35 subunit
• IL35p35
• interleukin 12, p35
• interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
• interleukin-12 alpha chain
• interleukin-12 subunit alpha
• NF cell stimulatory factor chain 1
• NFSK
• NK cell stimulatory factor chain 1
• NKSF1
• p35

Background

This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL12A