IL-12/IL-35 p35 Antibody (27537) [Phycoerythrin]

Images

 
Human peripheral blood mononuclear cells (PBMCs) either untreated (orange open histogram) or treated with LPS (orange filled histogram) were stained with Mouse Anti-Human/Mouse IL‑12/IL‑35 p35 PE‑conjugated ...read more
Mouse splenocytes either untreated (orange open histogram) or treated with LPS (orange filled histogram) were stained with Mouse Anti-Human/Mouse IL‑12/IL‑35 p35 PE‑conjugated Monoclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow
Clone
27537
Clonality
Monoclonal
Host
Mouse
Conjugate
Phycoerythrin

IL-12/IL-35 p35 Antibody (27537) [Phycoerythrin] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑12/IL-35 p35
Arg23-Ser219
Accession # P29459
Specificity
Detects human IL‑12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL12A
Purity
Protein A or G purified from ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells
Publications
Read Publications using
IC2191P in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide
Purity
Protein A or G purified from ascites

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-12/IL-35 p35 Antibody (27537) [Phycoerythrin]

  • IL-12/IL-35 p35
  • IL-12A
  • IL35p35

Background

Interleukin 12 (IL-12) and interleukin 35 (IL-35) are heterodimeric cytokines composed of alpha and beta chains. IL-12 is composed of p35 and p40 subunits, while IL-35 is comprised of p35 paired with EBI-3 (1). In mice, IL-35 is produced by FoxP3+ regulatory T cells and may function as an inhibitory cytokine to suppress T cell proliferation (2). Human FoxP3+ Tregs do not constitutively express IL-35 (3), but expression may be induced by activated dendritic cells (4).
  1. Collison, L.W. and D.A.A. Vignali (2008) Immunol. Rev. 226:248.
  2. Collison, L.W. et al. (2007) Nature 450:566.
  3. Bardel, E. et al. (2008) J. Immunol. 181:6898.
  4. Seyerl, M. et al.(2010) Eur. J. Immunol. 40:321.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-12/IL-35 p35 Antibody (IC2191P)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Flow Cytometry.


Filter By Application
Flow Cytometry
(6)
All Applications
Filter By Species
Human
(3)
Mouse
(3)
All Species
Showing Publications 1 - 6 of 6.
Publications using IC2191P Applications Species
AT Alshareeda, E Rakha, A Alghwainem, B Alrfaei, B Alsowayan, A Albugami, AM Alsubayyil, M Abomraee, NK Mohd Zin The effect of human placental chorionic villi derived mesenchymal stem cell on triple-negative breast cancer hallmarks PLoS ONE, 2018;13(11):e0207593. 2018 [PMID: 30458011] (Flow Cytometry, Human) Flow Cytometry Human
M Komorowski, J Tisonczyk, A Kolakowska, R Drozdz, D Kozbor Modulation of the Tumor Microenvironment by CXCR4 Antagonist-Armed Viral Oncotherapy Enhances the Antitumor Efficacy of Dendritic Cell Vaccines against Neuroblastoma in Syngeneic Mice Viruses, 2018;10(9):. 2018 [PMID: 30149659] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Marcin P Komorowski Reprogramming antitumor immunity against chemoresistant ovarian cancer by a CXCR4 antagonist-armed viral oncotherapy Mol Ther Oncolytics, 2016;3(0):16034. 2016 [PMID: 28035333] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Richter C, Thieme S, Bandola J, Laugsch M, Anastassiadis K, Brenner S Generation of inducible immortalized dendritic cells with proper immune function in vitro and in vivo. PLoS ONE, 2013;8(4):e62621. 2013 [PMID: 23626840] (Flow Cytometry, Mouse) Flow Cytometry Mouse
IL-35 over-expression increases apoptosis sensitivity and suppresses cell growth in human cancer cells. Biochem Biophys Res Commun, 2013;430(1):364-9. 2013 [PMID: 23154182] (Flow Cytometry, Human) Flow Cytometry Human
Chaturvedi V, Collison LW, Guy CS, Workman CJ, Vignali DA Cutting edge: Human regulatory T cells require IL-35 to mediate suppression and infectious tolerance. J. Immunol., 2011;186(12):6661-6. 2011 [PMID: 21576509] (Flow Cytometry, Human) Flow Cytometry Human

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Bioinformatics

Gene Symbol IL12A
Uniprot
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