IL-12/IL-35 p35 Antibody (27537) [Fluorescein]

• Applications: Flow
• Clone: 27537
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Fluorescein

IL-12/IL-35 p35 Antibody (27537) [Fluorescein] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑12/IL-35 p35
  Arg23-Ser219
  Accession # P29459
• Specificity: Detects human IL‑12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL12A
• Purity Statement: Protein A or G purified from ascites

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Interleukin 12 (IL-12) and interleukin 35 (IL-35) are heterodimeric cytokines composed of alpha and beta chains. IL-12 is composed of p35 and p40 subunits, while IL-35 is comprised of p35 paired with EBI-3 (1). In mice, IL-35 is produced by FoxP3⁺ regulatory T cells and may function as an inhibitory cytokine to suppress T cell proliferation (2). Human FoxP3⁺ Tregs do not constitutively express IL-35 (3), but expression may be induced by activated dendritic cells (4).

1. Collison, L.W. and D.A.A. Vignali (2008) Immunol. Rev. 226:248.
2. Collison, L.W. et al. (2007) Nature 450:566.
3. Bardel, E. et al. (2008) J. Immunol. 181:6898.
4. Seyerl, M. et al.(2010) Eur. J. Immunol. 40:321.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL12A