IL-12/IL-35 p35 Antibody (27537) [PerCP (Peridinin-chlorophyll Protein Complex)]

Images

 
Human peripheral blood mononuclear cells (PBMCs) either untreated (light orange filled histogram) or treated with LPS (dark orange filled histogram) were stained with Mouse Anti-Human/Mouse IL‑12/IL‑35 p35 ...read more
Mouse splenocytes either untreated (light orange filled histogram) or treated with LPS (dark orange filled histogram) were stained with Mouse Anti-Human/Mouse IL‑12/IL‑35 p35 PerCP‑conjugated Monoclonal Antibody ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow
Clone
27537
Clonality
Monoclonal
Host
Mouse
Conjugate
PerCP (Peridinin-chlorophyll Protein Complex)

IL-12/IL-35 p35 Antibody (27537) [PerCP (Peridinin-chlorophyll Protein Complex)] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑12/IL-35 p35
Arg23-Ser219
Accession # P29459
Specificity
Detects human IL‑12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL12A
Purity
Protein A or G purified from ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide
Purity
Protein A or G purified from ascites

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-12/IL-35 p35 Antibody (27537) [PerCP (Peridinin-chlorophyll Protein Complex)]

  • IL-12/IL-35 p35
  • IL-12A
  • IL35p35

Background

Interleukin 12 (IL-12) and interleukin 35 (IL-35) are heterodimeric cytokines composed of alpha and beta chains. IL-12 is composed of p35 and p40 subunits, while IL-35 is comprised of p35 paired with EBI-3 (1). In mice, IL-35 is produced by FoxP3+ regulatory T cells and may function as an inhibitory cytokine to suppress T cell proliferation (2). Human FoxP3+ Tregs do not constitutively express IL-35 (3), but expression may be induced by activated dendritic cells (4).
  1. Collison, L.W. and D.A.A. Vignali (2008) Immunol. Rev. 226:248.
  2. Collison, L.W. et al. (2007) Nature 450:566.
  3. Bardel, E. et al. (2008) J. Immunol. 181:6898.
  4. Seyerl, M. et al.(2010) Eur. J. Immunol. 40:321.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-12/IL-35 p35 Antibody (IC2191C) (0)

There are no publications for IL-12/IL-35 p35 Antibody (IC2191C).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for IL-12/IL-35 p35 Antibody (IC2191C) (0)

There are no reviews for IL-12/IL-35 p35 Antibody (IC2191C). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for IL-12/IL-35 p35 Antibody (IC2191C) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional IL-12/IL-35 p35 Products

Bioinformatics Tool for IL-12/IL-35 p35 Antibody (IC2191C)

Discover related pathways, diseases and genes to IL-12/IL-35 p35 Antibody (IC2191C). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Blogs on IL-12/IL-35 p35

There are no specific blogs for IL-12/IL-35 p35, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our IL-12/IL-35 p35 Antibody (27537) [PerCP (Peridinin-chlorophyll Protein Complex)] and receive a gift card or discount.

Bioinformatics

Gene Symbol IL12A
Uniprot
Novus 100% Guarantee